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Millipore/01-185 | VEGF Protein, Human recombinant/01-185/10 µg

價格: ￥4164.00 市場價: 6940.00

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貨號: 01-185

品牌: Millipore

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Description
CatalogueNumber	01-185
BrandFamily	Upstate
TradeName	Upstate
Description	VEGFProtein,Humanrecombinant

ProductInformation
Formulation
Presentation	10μgofVEGFlyophilizedproteinfrombuffercontaining25mMHEPESand150mMNaCl,pH7.0.Carrierfree.

StorageandShippingInformation
StorageConditions	Thelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewateror25mMHEPESand150mMNaCl,pH7.0.Reconstitutedmaterialcanbestoredat2°-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles. Thisproductisacidstable.Diluteineither0.1%TFAor4mMHCl,containing25ugBSAperugVEGFtomaintainstABIlity.Reducingreagents(suchasdithiothrietolor2-mercaptoethanol)inhibitVEGFactivity.

StorageandShippingInformation

StorageConditions

Thelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewateror25mMHEPESand150mMNaCl,pH7.0.Reconstitutedmaterialcanbestoredat2°-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles.

Thisproductisacidstable.Diluteineither0.1%TFAor4mMHCl,containing25ugBSAperugVEGFtomaintainstABIlity.Reducingreagents(suchasdithiothrietolor2-mercaptoethanol)inhibitVEGFactivity.

Applications
Application	ThehumanrecombinantVEGFProteinisavailableina10μgformat.
KeyApplications	CellCulture
ApplicationNotes	VEGFProliferationAssay 1.Priortouseinthisassay,maintainHUVEcellsinF-12Kmediumsupplementedwith10%(v/v)FBS,1%penicillin-streptomycin-glutamine,0.1mg/mlheparinand0.05mg/mlendothelialcellgrowthsupplement(ECGS). 2.ForassayofVEGF,subcultureHUVEcellsintoa96-wellplateatadensityof4,000cellsperwellinthegrowthmediumovernight. 3.AddVEGFtoafinalconcentrationrangeof0.2,0.4,0.8,1.6,3.2,6.2,12.5,25and50ng/ml.AddVEGFdiluenttoonesetofwellsasthenegativecontrol. 4.Fourdayslater,add20ulofPromegaSubstrateCellTiter96AqueousOneSolutionReagenttoeachwell. 5.Incubateat37?Cfor2hrsandreadatOD490nm.

BIOLOGicalInformation
Source	RecombinanthumanDNAexpressedinbaculovirusinfectedSf9cells
EntrezGeneNumber	NM_001025366.1 NM_001025367.1 NM_001025368.1 NM_001025369.1 NM_001025370.1 NM_001033756.1 NM_003376.4
EntrezGeneSummary	ThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms.
GeneSymbol	VEGFA VEGF-A VEGF VPF MGC70609
PurificationMethod	Sterilizedthrougha0.2micronmembranefilter
UniProtNumber	P15692
UniProtSummary	FUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145. SIZE:232aminoacids;27042Da SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity). SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin. TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon. SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily.
StemCellType	NeuralStemCells MesenchymalStemCells HematopoieticStemCells

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MaterialsInformation

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Millipore/01-185 | VEGF Protein, Human recombinant/01-185/10 µg

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