ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.
Brilliant Violet 421?
Amax: 407
Emax: 421nm
BD Brilliant™ Violet 421 (BV421) has an Excitation peak at 407 nm with maximal emission at 421 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
5-color imaging is possible with BV421, in combination with BV480, Alexa Fluor? 488, Rhodamine-Red™-X, and Alexa Fluor? 647. If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor? 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.
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