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Millipore/03-112 | RIPAb+ QKI-5/03-112/10 assays10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent

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貨號: 03-112

品牌: Millipore

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Description
CatalogueNumber	03-112
BrandFamily	Upstate
TradeName	RIPAb+ Upstate
Description	RIPAb+QKI-5
Overview	RIPAb+antibodiesareevaluatedusingtheRNABindingProteinImmunoprecipitation(RIP)assay.EachRIPAb+antibodysetincludesanegativecontrolantibodytoensurespecificityoftheRIPreactionandisverifiedfortheco-immunoprecipitationofRNAassociatedspecificallywiththeimmunoprecipitatedRNAbindingproteinofinterest.Whereappropriate,theRIPAb+setalsoincludesquantitativeRT-PCRcontrolprimers(RIPPrimers)toBIOLOGicallyvalidateyourIPresultsbysuccessfullyco-precipitatingthespecificRNAtargets,suchasmessengerRNAs.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateRIPprotocolswhenusingtheantibodyintheirexperimentalcontext.Ifatargetspecificassayisnotprovided,theRIPAb+kitisvalidatedusinganautomatedmicrofluidics-basedassaybyenrichmentofdetectableRNAovercontrolimmunoprecipitation.
AlternateNames	QuakingProtein
BackgroundInformation	QKIisanRNA-bindingproteinthatplaysacentralroleinmyelinization.Actsbyregulatingpre-mRNAsplicing,mRNAexport,mRNAstABIlityandproteintranslation.Itisregulatedbyalternativesplicing.

ProductInformation
Format	Purified
Control	IncludesnegativecontrolrabbitIgGantibodyandcontrolprimersspecificforhumanMAGI1.
Presentation	Anti-QKI-5(RabbitPolyclonal).Onevialcontaining50μLofrabbitserum.Storeat-20°C. NormalRabbitIgG.Onevialcontaining125μgRabbitIgGin125μLstoragebuffercontaining0.05%sodiumazide.Storeat-20°C. RIPPrimers,MAGI1.Onevialcontaining75μLof5μMofeachprimerspecificforhumanMAGI1.Storeat-20°C. FOR:TATTCCAAACCACCCTCCAA REV:CAACTCAGAAGGGTTTCGTGT

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
KeyApplications	RNABindingProteinImmunoprecipitation(RIP) Immunoprecipitation WesternBlotting
ApplicationNotes	ImmunoprecipitationfromRIPlysate: Representativelotdata. RIPlysatefromHeLacells(2X10⁶cellequivalentsperIP)wassubjectedtoimmunoprecipitationusing0.5μLofeitheranormalrabbitIgGor0.5μLofAnti-QKI-5antibody(Pleaseseefigures). AutomatedMicrofluidics-basedElectrophoreticRNASeparationandAnalysis: RIPLysatepreparedfromNTera-2cells(2X10⁷cellequivalentsperIP)weresubjectedtoimmunoprecipitationusing5μLofeitheranormalrabbitIgGor5μLofAnti-QKI-5antibodyandtheMagnaRIP?RNA-BindingProteinImmunoprecipitationKit(Cat.#17-700). SuccessfulimmunoprecipitationofQKI-5-associatedRNAwasverifiedbyautomatedmicrofluidics-basedelectrophoreticRNAseparationandanalysis. PleaserefertotheMagnaRIP?(Cat.#17-700)orEZ-MagnaRIP?(Cat.#17-701)protocolforexperimentaldetails. Electropherogramsweregeneratedbyplottingfluorescenceintensitiesversusmigrationtimes(Pleaseseefigures). Avirtualgelviewwascreatedfromthisdata(Pleaseseefigures). WesternBlotAnalysis:1:500-1:1,000dilutionofapreviouslotdetectedQKI-5inwesternblot.

Applications

KeyApplications

RNABindingProteinImmunoprecipitation(RIP)
Immunoprecipitation
WesternBlotting

ApplicationNotes

ImmunoprecipitationfromRIPlysate:
Representativelotdata.
RIPlysatefromHeLacells(2X10⁶cellequivalentsperIP)wassubjectedtoimmunoprecipitationusing0.5μLofeitheranormalrabbitIgGor0.5μLofAnti-QKI-5antibody(Pleaseseefigures).

AutomatedMicrofluidics-basedElectrophoreticRNASeparationandAnalysis:
RIPLysatepreparedfromNTera-2cells(2X10⁷cellequivalentsperIP)weresubjectedtoimmunoprecipitationusing5μLofeitheranormalrabbitIgGor5μLofAnti-QKI-5antibodyandtheMagnaRIP?RNA-BindingProteinImmunoprecipitationKit(Cat.#17-700).
SuccessfulimmunoprecipitationofQKI-5-associatedRNAwasverifiedbyautomatedmicrofluidics-basedelectrophoreticRNAseparationandanalysis.
PleaserefertotheMagnaRIP?(Cat.#17-700)orEZ-MagnaRIP?(Cat.#17-701)protocolforexperimentaldetails.

Electropherogramsweregeneratedbyplottingfluorescenceintensitiesversusmigrationtimes(Pleaseseefigures).
Avirtualgelviewwascreatedfromthisdata(Pleaseseefigures).

WesternBlotAnalysis:1:500-1:1,000dilutionofapreviouslotdetectedQKI-5inwesternblot.

BiologicalInformation
Immunogen	SyntheticpeptidecorrespondingtomouseQKI-5.
Host	Rabbit
Specificity	RecognizesQKI-5(QuakingProtein).
SpeciesReactivity	Human Mouse Rat
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_006766.1
EntrezGeneSummary	QKIbelongstoafamilyofRNA-bindingproteinsthathaveanHNRNPK(MIM600712)homology(KH)domainembeddedina200-aminoacidregioncalledtheGSGdomain.OthermembersofthisfamilyincludeSAM68(KHDRBS1;MIM602489)andSF1(MIM601516)(ChenandRichard,1998[PubMed9671495]).[suppliedbyOMIM]
GeneSymbol	QKI DKFZp586I0923 Hqk QK3 QK HKQ HqkI
PurificationMethod	Serum
UniProtNumber	Q96PU8
UniProtSummary	FUNCTION:SwissProt:Q96PU8#RNA-bindingproteinthatplaysacentralroleinmyelinization.Bindstothe5"-NACUAAY-N(1,20)-UAAY-3"RNAcoresequence.Actsbyregulatingpre-mRNAsplicing,mRNAexport,mRNAstabilityandproteintranslation.RequiredtoprotectandpromotestabilityofmRNAssuchasMBPandCDKN1Bwhichpromotesoligodendrocytedifferentiation.ParticipatesinmRNAtransportbyregulatingthenuclearexportofMBPmRNA.AlsoinvolvedinregulationofmRNAsplicingofMAGpre-mRNA.Actsasatranslationalrepressor(Bysimilarity). SIZE:341aminoacids;37671Da SUBUNIT:Homodimer.DoesnotrequireRNAtohomodimerize.AbletoheterodimerizewithBICC1(Bysimilarity). SUBCELLULARLOCATION:Nucleus(Bysimilarity).Cytoplasm(Bysimilarity). TISSUESPECIFICITY:Expressedinthefrontalcortexofbrain. PTM:MethylatedbyPRMT1(Bysimilarity).&TyrosinephosphorylatedatitsC-terminus,probablybyFYN.PhosphorylationleadstodecreasedmRNA-bindingaffinity,affectingtransportand/orstabilizationofMBPmRNA(Bysimilarity). DISEASE:SwissProt:Q96PU8#Mayplayaroleingeneticsusceptibilitytoschizophrenia.QKIlevelsofsomeisoformssuchasisoform6,arefrequentlydown-regulatedinschizophrenicpatients.&DeletionoftheQKIgenemayplayaroleinastrocytictumors. SIMILARITY:SwissProt:Q96PU8##Contains1KHdomain.
MolecularWeight	~40kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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Millipore/03-112 | RIPAb+ QKI-5/03-112/10 assays10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent

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