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            當前位置 : Millipore >>> Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL
            Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL
            • Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL

            Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL

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            貨號: 04-349
            品牌: Millipore
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              • Description
                CatalogueNumber04-349
                DescriptionAnti-MAPKinase2/Erk2Antibody,cloneE460,rabbitmonoclonal
                AlternateNames
                • mitogen-activatedproteinkinase1
                • Extracellularsignal-regulatedkinase2
                • Mitogen-activatedproteinkinase2
                • MAPkinase1
                • MAPkinase2
                • MAPkinaseisoformp42
                • proteintyrosinekinaseERK2
                BackgroundInformationExtracellularsignalregulatedkinase2(ERK2)alsoknownasmaturationormitogenactivatedproteinkinase(MAPK)isinvolvedineukaryoticsignaltransduction(1).Activatedbyadualphosphorylationonthreonineandtyrosineresiduebythedual-specificitykinaseMKK1/2,ERK2p42translocatetothenucleusafterstimulation(2).Oncephosphorylated,ERK2canformdimerswithphosphorylatedandunphosphorylatedERK2partners.ItisalsoabletophosphorylatemultiplesubstratessuchasEGFR,c-PLA2,Elk1/TCFandc-Myc(3-5).
                ProductInformation
                FormatUnpurified
                Control
                • A431celllysate
                PresentationRabbitMonoclonalinbuffercontainingglycerol,BSA,andsodiumazide.
                StorageandShippingInformation
                StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
                HandlingRecommendations:Uponfirstthaw,andpriortoremovingcap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20oC.Avoidfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
                Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
                Applications
                ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.
                KeyApplications
                • WesternBlotting
                • Immunocytochemistry
                • Immunoprecipitation
                • FlowCytometry
                • Immunohistochemistry(Paraffin)
                ApplicationNotesImmunohistochemistryAnalysis:A1:250-500dilutionfromarepresentativelotwasusedinhumanbreastcarcinomatissue.
                ImmunocytochemistryAnalysis:A1:100-250dilutionfromarepresentativelotwasusedinIC.
                ImmunoprecipitationAnalysis:A1:80dilutionfromarepresentativelotwasusedinIP.
                FlowCytometryAnalysis:A1:50dilutionfromarepresentativelotwasusedinFC.
                BIOLOGicalInformation
                ImmunogenSyntheticpeptidecorrespondingtotheC-terminusofhumanMAPKinase2/Erk2.
                EpitopeC-terminus
                CloneE460
                HostRabbit
                IsotypeIgG
                SpeciesReactivity
                • Human
                • Mouse
                • Rat
                AntibodyTypeMonoclonalAntibody
                EntrezGeneNumber
                GeneSymbol
                • ERK2
                • PRKM1
                • ERK
                • MAPK2
                • p41mapk
                • ERK-2
                • ERT1
                • PRKM2
                • MAPK2
                • p42-MAPK
                • EC2.7.11.24
                • OTTHUMP00000028754
                • OTTHUMP00000174492
                • P42MAPK
                • p38
                • p40
                • p41
                • MAPK1
                • EC2.7.11
                PurificationMethodUnpurified
                UniProtNumber
                UniProtSummaryFUNCTION:Serine/threoninekinasewhichactsasanessentialcomponentoftheMAPkinasesignaltransductionpathway.MAPK1/ERK2andMAPK3/ERK1arethe2MAPKswhichplayanimportantroleintheMAPK/ERKcascade.TheyparticipatealsoinasignalingcascadeinitiatedbyactivatedKITandKITLG/SCF.Dependingonthecellularcontext,theMAPK/ERKcascademediatesdiversebiologicalfunctionssuchascellgrowth,adhesion,survivalanddifferentiationthroughtheregulationoftranscription,translation,cytoskeletalrearrangements.ThetheMAPK/ERKcascadeplaysalsoaroleininitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactors.About160substrateshavealreadybeendiscoveredforERKs.Manyofthesesubstratesarelocalizedinthenucleus,andseemtoparticipateintheregulationoftranscriptionuponstimulation.However,othersubstratesarefoundinthecytosolaswellasinothercellularorganelles,andthoseareresponsIBLeforprocessessuchastranslation,mitosisandapoptosis.Moreover,theMAPK/ERKcascadeisalsoinvolvedintheregulationoftheendosomaldynamics,includinglysosomeprocessingandendosomecyclingthroughtheperinuclearrecyclingcompartment(PNRC);aswellasinthefragmentationoftheGolgiapparatusduringmitosis.Thesubstratesincludetranscriptionfactors(suchasATF2,BCL6,ELK1,ERF,FOS,HSF4orSPZ1),cytoskeletalelements(suchasCANX,CTTN,GJA1,MAP2,MAPT,PXN,SORBS3orSTMN1),regulatorsofapoptosis(suchasBAD,BTG2,CASP9,DAPK1,IER3,MCL1orPPARG),regulatorsoftranslation(suchasEIF4EBP1)andavarietyofothersignaling-relatedmolecules(likeARHGEF2,FRS2orGRB10).Proteinkinases(suchasRAF1,RPS6KA1/RSK1,RPS6KA3/RSK2,RPS6KA2/RSK3,RPS6KA6/RSK4,SYK,MKNK1/MNK1,MKNK2/MNK2,RPS6KA5/MSK1,RPS6KA4/MSK2,MAPKAPK3orMAPKAPK5)andphosphatases(suchasDUSP1,DUSP4,DUSP6orDUSP16)areothersubstrateswhichenablethepropagationtheMAPK/ERKsignaltoadditionnalcytosolicandnucleartargets,therebyextendingthespecificityofthecascade.
                Actsasatranscriptionalrepressor.Bindstoa[GC]AAA[GC]consensussequence.Represstheexpressionofinterferongamma-inducedgenes.SeemstobindtothepromoterofCCL5,DMP1,IFIH1,IFITM1,IRF7,IRF9,LAMP3,OAS1,OAS2,OAS3andSTAT1.Transcriptionalactivityisindependentofkinaseactivity.
                CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein.
                COFACTOR:MagnesiumBysimilarity.
                ENZYMEREGULATION:PhosphorylatedbyMAP2K1/MEK1andMAP2K2/MEK2onThr-185andTyr-187inresponsetoexternalstimulilikeinsulinorNGF.Bothphosphorylationsarerequiredforactivity.Thisphosphorylationcausesdramaticconformationalchanges,whichenablefullactivationandinteractionofMAPK1/ERK2withitssubstrates.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DephospphorylatedandinactivatedbyDUSP3,DUSP6andDUSP9.Inactivatedbypyrimidylpyrroleinhibitors.
                SUBUNITSTRUCTURE:Bindsbothupstreamactivatorsanddownstreamsubstratesinmultimolecularcomplexes.BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithADAM15,ARHGEF2,ARRB2,DAPK1(viadeathdomain),HSF4,IER3,IPO7,DUSP6,NISCH,SGK1,andisoform1ofNEK2.Interacts(phosphorylatedform)withCAV2("Tyr-19"-phosphorylatedform);theinteraction,promotedbyinsulin,leadstonuclearlocationandMAPK1activation.InteractswithMORG1,PEA15andMKNK2Bysimilarity.MKNK2isoform1bindingpreventsfromdephosphorylationandinactivationBysimilarity.
                SUBCELLULARLOCATION:Nucleus.Cytoplasm?Cytoskeleton?centrosome.Cytoplasm.Note:PEA15-bindingandphosphorylatedDAPK1promoteitscytoplasmicretention.PhosphorylationatSer-246andSer-248aswellasautophosphorylationatThr-190promotenuclearlocalization.
                DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases.
                PTM:PhosphorylateduponKITandFLT3signalingBysimilarity.DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme.UndergoesregulatoryphosphorylationonadditionalresiduessuchasSer-246andSer-248inthekinaseinsertdomain(KID)Thesephosphorylations,whichareprobablymediatedbymorethanonekinase,areimportantforbindingofMAPK1/ERK2toimportin-7(IPO7)anditsnucleartranslocation.Inaddition,autophosphorylationofThr-190wasshowntoaffectthesubcellularlocalizationofMAPK1/ERK2aswell.Ligand-activatedALKinducestyrosinephosphorylation.DephosphorylatedbyPTPRJatTyr-187.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DUSP3andDUSP6dephosphorylatespecificallyMAPK1/ERK2andMAPK3/ERK1whereasDUSP9dephosphorylatesabroaderrangeofMAPKs.
                SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.
                Contains1proteinkinasedomain.
                SEQUENCECAUTION:ThesequenceCAA77753.1differsfromthatshown.Reason:Erroneousinitiation.TranslationN-terminallyextended.
                MolecularWeight~42kDaobserved
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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