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Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg

價格: ￥4680.00 市場價: 7800.00

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貨號: 05-710

品牌: Millipore

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Description
CatalogueNumber	05-710
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-NG2Antibody,clone132.38

ProductInformation
Format	Purified
Control	Braintissue
Presentation	ProteinAPurifiedimmunoglobulinin0.1MTris-glycine,pH7.4,0.15MNaClwith0.05%sodiumazideasapreservative.

StorageandShippingInformation
StorageConditions	Maintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

Applications
Application	Anti-NG2Antibody,clone132.38detectslevelofNG2&hasbeenpublished&validatedforuseinIH&WB.
KeyApplications	Immunohistochemistry WesternBlotting
ApplicationNotes	Cross-reactivityexpectedwithmouse.

BIOLOGicalInformation
Immunogen	HEK293cellsexpressingatruncatedintegralmembraneformofNG2consistingofaminoacids1592-2222
Clone	132.38
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	RecognizesNG2.
Isotype	IgG1
SpeciesReactivity	Mouse Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_001897
EntrezGeneSummary	Ahumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreADIngonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells.
GeneSymbol	CSPG4 MCSP HMW-MAA MCSPG MSK16 NG2 MEL-CSPG
PurificationMethod	ProteinGPurified
UniProtNumber	Q6UVK1
UniProtSummary	FUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades. SIZE:2322aminoacids;250497Da SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16. SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity). TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells. PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity). SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains. MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells.
MolecularWeight	~270-300kDa

PhysicochemicalInformation

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MaterialsInformation

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Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg

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