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Millipore/05-716 | Anti-Myosin Heavy Chain Antibody, clone A4.1025/05-716/200 µL

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貨號: 05-716

品牌: Millipore

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Description
CatalogueNumber	05-716
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-MyosinHeavyChainAntibody,cloneA4.1025
AlternateNames	Myosinheavychain1 Myosinheavychain2x MyosinheavychainIIx/d Myosinheavychain,skeletalmuscle,adult1 myosin,heavychain1,skeletalmuscle,adult myosin,heavypolypeptide1,skeletalmuscle,adult
BackgroundInformation	MyosinisoneofthetwomajorcomponentsofmuscletissueresponsIBLeforcontraction.Inmusclecellsmyosinisarrangedasthickfilamentousstructuresthatarearrangedinparalleltothinfilamentousstructurescomposedofactin.MusclecontractionisactuatedbythecouplinganduncouplingofthinandthickfilamentsthroughachemicallinkagemediatedbyATP.Thecouplinganduncouplingisarrangedsuchthatthefilamentsslideinonedirectionwhencoupledandinreversedirectionwhenuncoupledorreleased.Myosinandactinalsoservetofacilitatemotilityinnon-musclecellssuchasinslimemoldswhereshorteryetsimilarfilamentousstructuresworktogether.Anincreasingnumberofdifferentmyosinsarebeingdescribed.MyosinIisalowmolecularweightmyosinfoundinprotozoaanddoesnotself-assemble.MyosinIIistheclassicformfoundinmusclecellsthatselfassembleintothickbundles.MyosinIIisamultimericproteinthatiscomposedoftwoheavychainsandtwolightchains.

ProductInformation
Format	Ascites
Control	RIPAlysatesfromC2C12cells
Presentation	UnpurifiedmouseascitesmonoclonalIgGinbuffercontaining0.07MTris-glycine,pH7.4,0.105MNaCl,0.035%sodiumazideand30%glycerol.Liquidat-20°C.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20oCfromdateofreceipt. Formaximumrecoveryofproduct,centrifugethevialpriortoremovingthecap. HandlingRecommendations: Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerol-containingsolutionstobecomefrozenduringstorage.

StorageandShippingInformation

StorageConditions

Stablefor1yearat-20oCfromdateofreceipt.
Formaximumrecoveryofproduct,centrifugethevialpriortoremovingthecap.

HandlingRecommendations:
Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerol-containingsolutionstobecomefrozenduringstorage.

Applications
Application	Anti-MyosinHeavyChainAntibody,cloneA4.1025isanantibodyagainstMyosinHeavyChainforuseinIH&WB.
KeyApplications	Immunohistochemistry WesternBlotting
ApplicationNotes	Immunohistochemistry: Thisantibodyhasbeenreportedbyanindependentlaboratorytodetectmyosinheavychaininzebrafishembryosandskeletalmusclesectionsfixedwith4%paraformaldehydeinapreviouslot(Blagden,C.S.,1997;Cho,M.,1994).

BIOLOGicalInformation
Immunogen	Partiallypurifiedproteinfromadulthumanskeletalmusclemyosin.CloneA4.1025.
Clone	A4.1025
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	Thisantibodyrecognizesmyosinheavychain,Mr~220kDa.Immunoblottingwithmusclecelllysateswilloftenshowseveralclosebandsfromdifferentmyosinheavychaingenes,aswellasdegradationproducts.
Isotype	IgG
SpeciesReactivity	Human Rat Mouse Zebrafish Drosophila Rabbit
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_005963
EntrezGeneSummary	MyosinisamajorcontractileproteinwhichconvertschemicalenergyintomechanicalenergythroughthehydrolysisofATP.Myosinisahexamericproteincomposedofapairofmyosinheavychains(MYH)andtwopairsofnonidenticallightchains.Myosinheavychainsareencodedbyamultigenefamily.Inmammalsatleast10differentmyosinheavychain(MYH)isoformshavebeendescribedfromstriated,smooth,andnonmusclecells.Theseisoformsshowexpressionthatisspatiallyandtemporallyregulatedduringdevelopment.
GeneSymbol	MYH1 MyHC-2X/D MGC133384 MYHa MyHC-IIx/d MyHC-2x Myosin-1 MYHSA1
PurificationMethod	Unpurified
UniProtNumber	P12882
UniProtSummary	FUNCTION:SwissProt:P12882#Musclecontraction. SIZE:1939aminoacids;223115Da SUBUNIT:Musclemyosinisahexamericproteinthatconsistsof2heavychainsubunits(MHC),2alkalilightchainsubunits(MLC)and2regulatorylightchainsubunits(MLC-2). SUBCELLULARLOCATION:Cytoplasm,myofibril.Note=Thickfilamentsofthemyofibrils. DOMAIN:SwissProt:P12882Therodliketailsequenceishighlyrepetitive,showingcyclesofa28-residuerepeatpatterncomposedof4heptapeptides,characteristicforalpha-helicalcoiledcoils. SIMILARITY:Contains1IQdomain.&Contains1myosinhead-likedomain. MISCELLANEOUS:Eachmyosinheavychaincanbesplitinto1lightmeromyosin(LMM)and1heavymeromyosin(HMM).Itcanlaterbesplitfurtherinto2globularsubfragments(S1)and1rod-shapedsubfragment(S2).
MolecularWeight	~220kDa

PhysicochemicalInformation

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Millipore/05-716 | Anti-Myosin Heavy Chain Antibody, clone A4.1025/05-716/200 µL

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