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Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg

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貨號: 05-806

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Description
CatalogueNumber	05-806
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-phospho-HistoneH3(Ser10)Antibody,clone3H10
AlternateNames	H3histone,family3A H3S10P HistoneH3(phosphoS10) H3histone,family3A H3histone,family3B H3histone,family3B(H3.3B)
BackgroundInformation	HistoneH3isoneofthefivemainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongN-terminaltail,H3isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.TheN-terminaltailofhistoneH3protrudesfromtheglobularnucleosomecoreandcanundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses.Thesemodificationsincludethecovalentattachmentofmethyloracetylgroupstolysineandarginineaminoacidsandthephosphorylationofserineorthreonine.

ProductInformation
Format	Purified
Control	UV-treated293cellextracts,UV-treatedHeLacellextracts,breastcancertissue,HEPG2cellextracts.
Presentation	PurifiedmouseIgG1kinbuffercontaining0.1MTris-glycine,pH7.4,0.15MNaCland0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2to8°Cfromdateofreceipt. HandlingRecommendations: Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	Anti-phospho-HistoneH3(Ser10)Antibody,clone3H10isamousemonoclonalantibodyfordetectionofHistoneH3phosphorylatedatserine10.ThispurifiedmAb,alsoknownasH3S10p,ispublishedinpeerreviewedjournalsandhasbeenvalidatedinFC,ICC,IF,PIA,WB,Mplex.
KeyApplications	FlowCytometry Immunocytochemistry Immunofluorescence PeptideInhibitionAssay WesternBlotting Multiplexing
ApplicationNotes	FlowCytometry: ThisantibodyhasbeenreportedbyanindependentlaboratorytodetectphosphorylatedhistoneH3usingflowcytometry. Beadlyte?Histone-PeptideSpecificityAssay: 1:1,000to1:81,000dilutionsofapreviouslotwereincubatedwithhistoneH3peptidescontainingvariousmodificationsconjugatedtoLuminex?microspheres.(FigureB).Onlythepeptidecontainingphospho-serine10wasdetected. Immunocytochemistry: 0.2μg/mLofapreviouslotshowedpositivechromosomeimmunostainingformitoticA431andHeLacellsfixedwith95%ethanoland5%aceticacidandpermeabilizedwith0.1%Triton?X-100. PeptideInhibitionAnalysis: DetectionofhistoneH3inimmunoblotsofcolcemidtreatedHeLaacidextractsbyanti-phospho-HistoneH3(Ser10)wasdiminishedby10μMofhistoneH3peptidecontainingphospho-serine10,butnotbypeptidescontainingphospho-serine28oranunmodifiedhistoneH3sequence(FigureC). Immunofluorescence:

BIOLOGicalInformation
Immunogen	Aproprietaryimmunogenbasedonapeptidesequencecontainingphospho-serinecorrespondingtoresidue10ofhumanhistoneH3.
Clone	3H10
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	HistoneH3phosphorylatedatSer10.
Isotype	IgG1κ
SpeciesReactivity	Human
SpeciesReactivityNote	Human. Broadspeciescross-reactivityisexpected.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_003493
EntrezGeneSummary	HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH3family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.ThisgeneislocatedseparatelyfromtheotherH3genesthatareinthehistonegeneclusteronchromosome6p22-p21.3.
GeneSymbol	HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ HIST3H3 H3FT H3T MGC126886
Modifications	Phosphorylation
PurificationMethod	ProteinGPurified
UniProtNumber	Q16695
UniProtSummary	FUNCTION:SwissProt:Q16695#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:136aminoacids;15508Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. TISSUESPECIFICITY:Expressedintesticularcells. DEVELOPMENTALSTAGE:ExpressedduringSphase,thenexpressionstronglydecreasesascelldivisionslowsdownduringtheprocessofdifferentiation. PTM:Acetylationisgenerallylinkedtogeneactivation.AcetylationonLys-10impairsmethylationatArg-9.AcetylationonLys-19andLys-24favorsmethylationatArg-18(Bysimilarity).&CitrullinationatArg-9and/orArg-18byPADI4impairsmethylationandrepressestranscription(Bysimilarity).&AsymmetricdimethylationatArg-18byCARM1islinkedtogeneactivation.SymmetricdimethylationatArg-9byPRMT5islinkedtogenerepression(Bysimilarity).&MethylationatLys-5,Lys-37andLys-80arelinkedtogeneactivation.MethylationatLys-5facilitatessubsequentacetylationofH3andH4.MethylationatLys-80isassociatedwithDNAdouble-strandbreak(DSB)responsesandisaspecifictargetforTP53BP1.MethylationatLys-10andLys-28arelinkedtogenerepression.MethylationatLys-10isaspecifictargetforHP1proteins(CBX1,CBX3andCBX5)andpreventssubsequentphosphorylationatSer-11andacetylationofH3andH4.MethylationatLys-5andLys-80requirepreliminarymonoubiquitinationofH2Bat"Lys-120".MethylationatLys-10andLys-28areenrichedininactiveXchromosomechromatin(Bysimilarity).&PhosphorylatedatThr-4byGSG2/haspinduringprophaseanddephosphorylatedduringanaphase.Atcentromeres,specificallyphosphorylatedatThr-12fromprophasetoearlyanaphase.PhosphorylatedatSer-11duringthewholemitosis.PhosphorylationatSer-11,whichislinkedtogeneactivation,preventsmethylationatLys-10butfacilitatesacetylationofH3andH4.PhosphorylatedatSer-29byMLTKisoform1,RPS6KA5orAURKBduringmitosisoruponultravioletBirradiation(Bysimilarity).&Phosphorylationat"Ser-11"iscrucialforchromosomecondensationandcell-cycleprogressionduringmitosisandmeiosis.Inadditionphosphorylationat"Ser-11"isimportantduringinterphasebecauseitenablesthetranscriptionofgenesfollowingexternalstimulation,likestressorgrowthfactors.Phosphorylationat"Ser-11"isalsoanessentialregulatorymechanismforneoplasticcelltransformation.Phosphorylationat"Ser-11"byAURKB/Aurora-BmediatesthedissociationofHP1proteins(CBX1,CBX3andCBX5)fromheterochromatin.&Ubiquitinated(Bysimilarity). SIMILARITY:SwissProt:Q16695##BelongstothehistoneH3family.
MolecularWeight	17kDa

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Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg

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