| Immunogen | Inordertoproducebroadspectrumpolyclonalphosphotyrosineantibodies,rabbitswereimmunizedwiththreephosphorylatedimmunogens:(1)phosphotyrosinecovalentlylinkedtoKLH;(2)thec-Srccarboxylterminalregulatoryphosphopeptide(TS-T-E-P-Q-pY-Q-P-G-E-N-L;Catalog#12-218)covalentlylinkedtoKLH,and;(3)aphosphopeptideassociatedwithhightyrosinekinaseactivityinhumanlymphocytes(R-R-L-I-E-D-A-E-pY-A-A-R-G;Catalog#12-217)covalentlylinkedtoKLH.BothofthephosphopeptidehaptensserveasstrongsubstratesfortyrosinephosphatasesandarepartofthetwocolorimetrictyrosinephosphatasekitsprovidedbyUpstate,Inc.(Catalog#17-125,17-126). | 
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. | 
| Host | Rabbit | 
| Specificity | Theimmunoreactivityoftheantibodyistotallyinhibitedbytheuseof100mMphenylphosphate,aphosphotyrosineanalog.Thephosphotyrosineantibodyispurifiedbyimmunoaffinitychromatographyusingeitheradualphospho-peptidegeloraBSA-phosphotyrosinegel.Allofthephosphotyrosineimmunoreactivitypresentintheantiseraisimmunoadsorbedwhethertheantibodyispurifiedbyeithergelindicatingthattheantibodyisnotsequence-specificbutspecificforphosphotyrosineresidues. | 
| SpeciesReactivity |  | 
| SpeciesReactivityNote | Human.PredictedtoreactwithAllbasedon100%sequencehomology. | 
| AntibodyType | PolyclonalAntibody | 
| Modifications |  | 
| PurificationMethod | AffinityPurfied | 
| MolecularWeight | Varies |