當前位置： Millipore >>> Millipore/06-847 | Anti-EGFR Antibody/06-847/200 µg

Millipore/06-847 | Anti-EGFR Antibody/06-847/200 µg

價格: 試用市場價: 0.00

打印產品信息

貨號: 06-847

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即申請

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	06-847
Replaces	04-337;04-338
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-EGFRAntibody
AlternateNames	Receptortyrosine-proteinkinaseErbB-1 avianerythroblasticleukemiaviral(v-erb-b)oncogenehomolog cellgrowthinhibitingprotein40 cellproliferation-inducingprotein61 epidermalgrowthfactorreceptor epidermalgrowthfactorreceptor(avianerythroblasticleukemiaviral (v-erb-b)oncogenehomolog) epidermalgrowthfactorreceptor(erythroblasticleukemiaviral(v-erb-b)oncogenehomolog,avian)
BackgroundInformation	Theepidermalgrowthfactorreceptor(EGFR;ErbB-1;HER1inhumans)isthecell-surfacereceptorformembersoftheepidermalgrowthfactorfamily(EGF-family)ofextracellularproteinligands.TheepidermalgrowthfactorreceptorisamemberoftheErbBfamilyofreceptors,asubfamilyoffourcloselyrelatedreceptortyrosinekinases:EGFR(ErbB-1),HER2/c-neu(ErbB-2),Her3(ErbB-3)andHer4(ErbB-4).MutationsaffectingEGFRexpressionoractivitycouldresultincancer.EGFR(epidermalgrowthfactorreceptor)existsonthecellsurfaceandisactivatedbybindingofitsspecificligands,includingepidermalgrowthfactorandtransforminggrowthfactorα(TGFα).Uponactivationbyitsgrowthfactorligands,EGFRundergoesatransitionfromaninactivemonomericformtoanactivehomodimer.Inadditiontoforminghomodimersafterligandbinding,EGFRmaypairwithanothermemberoftheErbBreceptorfamily,suchasErbB2/Her2/neu,tocreateanactivatedheterodimer.EGFRdimerizationstimulatesitsintrinsicintracellularprotein-tyrosinekinaseactivity.Asaresult,autophosphorylationoffivetyrosine(Y)residuesintheC-terminaldomainofEGFRoccurs.

ProductInformation
Format	Purified
Control	A431celllysate,humancervicalcarcinoma. IncludedPositiveAntigenControl: Catalog#12-305,3T3/A31CellLysate.Add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
Presentation	PurifiedrabbitpolyclonalIgGinbuffercontaining0.1MTris-Glycine,0.15MNaCl,0.05%SodiumAzide,pH7.4.Storeat2-8°C.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8°Cfromdateofreceipt. HandlingRecommendations:Uponfirstthaw, andpriortoremovingthecap,centrifugethe vialandgentlymixthesolution.

Applications
Application	Anti-EGFRAntibodydetectslevelofEGFR&hasbeenpublished&validatedforuseinIP&WB.
KeyApplications	Immunoprecipitation WesternBlotting
ApplicationNotes	Immunoprecipitation: 4μgofapreviouslotimmunoprecipitatedtheEGFRfrom500μgofa3T3/A31RIPAcelllysate.

BIOLOGicalInformation
Immunogen	Ovalbuminconjugatedsyntheticpeptide(ETKPNGIFKGPTAENAEYLRVAPPSSEFIGA)correspondingtoaminoacids1156-1186oftheprocessedmouseEGFreceptor(EGFR)C-terminaldomain(AccessionnumberQ01279).Theimmunizingsequenceshares28/31identitywiththehumanEGFreceptorsequence.
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	RecognizestheEGFR,Mr180kDa.
Isotype	IgG
SpeciesReactivity	Human Mouse Rat Hamster
SpeciesReactivityNote	Mouseandhuman.Reportedtodetectratandhamster.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_005219
EntrezGeneSummary	Theproteinencodedbythisgeneisatransmembraneglycoproteinthatisamemberoftheproteinkinasesuperfamily.Thisproteinisareceptorformembersoftheepidermalgrowthfactorfamily.EGFRisacellsurfaceproteinthatbindstoepidermalgrowthfactor.Bindingoftheproteintoaligandinducesreceptordimerizationandtyrosineautophosphorylationandleadstocellproliferation.Mutationsinthisgeneareassociatedwithlungcancer.[providedbyRefSeq]
GeneSymbol	EGFR ERBB1 ERBB mENA
PurificationMethod	ProteinAchromatography
UniProtNumber	P00533
UniProtSummary	FUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST. SIZE:1210aminoacids;134277Da SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted. TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers. PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain. MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation.
MolecularWeight	180kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
￥830.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥830.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00
Parafilm M封口膜/PM996/4x125/整箱
￥1296.00

Millipore/06-847 | Anti-EGFR Antibody/06-847/200 µg

客戶服務

何為188

關注188微信公眾號