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Millipore/06-863 | Anti-CREB Antibody/06-863/200 µg

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貨號: 06-863

品牌: Millipore

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Description
CatalogueNumber	06-863
Replaces	04-218
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-CREBAntibody
AlternateNames	CyclicAMP-responsiveelement-bindingprotein1 cAMP-responsiveelement-bindingprotein1 CREB-1
BackgroundInformation	CyclicAMP-responsiveelement-bindingprotein1(UniProtP16220;alsoknownascAMP-responsiveelement-bindingprotein1,CREB-1)isencodedbytheCREB1gene(GeneID1385)inhuman.CREBisatranscriptionfactorthatbindsthecAMPresponseelements(CRE)andregulatesthetranscriptionofthedownstreamgenes.CREBactivityisregulationviaphosphorylationbyvariouskinases,includingPKA,andCa2+/calmodulin-dependentproteinkinases.GenesknowntoberegulatedbyCREBincludec-fos,BDNF,tyrosinehydroxylase,neuropeptides(suchassomatostatin,enkephalin,VGF,andcorticotropin-releasinghormone),aswellasthecircADIanclockgenesPER1andPER2.CREBiscriticalformemoryformationacrossawiderangeofspecies.

ProductInformation
Format	ProteinAPurified
Control	HeLanuclearextract IncludedPositiveAntigenControl:Catalog#12-309,HeLanuclearextract.AddanequalvolumeofLaemmlireducingsamplebufferto5-10μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
Presentation	Purifiedrabbitpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidewith30%glycerol.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	Anti-CREBAntibody,Cat.No.06-863,isahighlyspecificrabbitPolyclonalantibody,thattargetsCREBandhasbeentestedinChromatinImmunoprecipitation(ChIP),ChIP-seq,ElectrophoreticMobilityShiftAssay(EMSA),Immunoprecipitation,andWesternBlotting.
KeyApplications	WesternBlotting Immunoprecipitation ChromatinImmunoprecipitation(ChIP) ChIP-seq ElectrophoreticMobilityShiftAssay
ApplicationNotes	ImmunoprecipitationAnalysis:10μgfromarepresentativelotimmunoprecipitatedCREBin500μgofHeLanuclearextract. ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedanenhancedCREBoccupancyattheLYPD3promoterinLKB1-deficientTE-4humanesophagealcancercells(Gu,Y.,etal.(2012).Oncogene.31(4):469–479). ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedCREBassociationattheRNASET2sitestargetedbyT-cellLeukemiaVirustype1(HTLV-1)encodedTaxproteinusingHTLV-1-infectedhumanT-celllines,MT-2,C8166/45andSLB-1(Polakowski,N.,etal.(2011).Viruses.3(8):1485-500). ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedCREBoccupancyatthehBDNFpromoterIVusingpostmortemhumanparietalcortextissuechromatinpreparations(Pruunsild,P.,etal.(2011).J.Neurosci.31(9):3295-3308). ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedCREBoccupancyatthemiR-9-2promoterinSK-N-BEhumanneuroblastomacells(Laneve,P.,etal.(2010).Nucl.AcidsRes.38(20):6895-6905). ChIP-seqAnalysis:ArepresentativelotidentifiedCREBtargetgenesinmurinespermatogoniaderivedGC1-spgcellsbyChIP-seqanalysis(Martianov,I.,etal.(2010).BMCGenomics.11:530). WesternBlottingAnalysis:RepresentativelotsdetectedCREBinlysatesfromculturedembryonicratstriatalneuronsandPC12pheochromocytomacells(Hutchinson,A.N.,etal.(2012).Neuropsychopharmacology.37(2):321-337;Maharjan,S.,etal.(2010).J.NeuRochem.112(1):42-55). WesternBlottingAnalysis:RepresentativelotsdetectedCREBinmouseproximalcaputepididymis-1(PC-1)celllysateandhippocampustissuehomogenates(Hamzeh,M.,andRobaire,B.(2011).J.Endocrinol.209(1):55-64;Zhou,Z.,etal.(2009).Neuropharmacology.56(1):81-89). WesternBlottingAnalysis:ArepresentativelotdetectedCREBinSK-N-BEhumanneuroblastomacelllysate(Laneve,P.,etal.(2010).Nucl.AcidsRes.38(20):6895-6905). ElectrophoreticMobilityShiftAssay(EMSA):ArepresentativelotcausedasupershiftofDNA-CREBcomplexinEMSAusingradiolabeledoligonucleotidecontaininghBDNFpromoterICREB-bindingsequenceandlysatesfromKCl-treatedprimaryratneurons(Pruunsild,P.,etal.(2011).J.Neurosci.31(9):3295-3308).

BIOLOGicalInformation
Immunogen	KLH-conjugated,syntheticpeptide(CSGAENQQSGDAAVTEAENQQ)correspondingtoa.a.5-24ofhumanCREB.Theimmunizingsequenceisidenticalinpig,shares19of20residueswithmouseandbovineand18of20residueswithrat.
Epitope	Aminoacids5-24.
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	ThisantibodyrecognizesCREB.
Isotype	IgG
SpeciesReactivity	Human Mouse Rat
SpeciesReactivityNote	Human,Mouse,Rat.PredictedtoreactwithCanine,Horse,Porcinebasedon100%sequencehomology.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_604391
EntrezGeneSummary	ThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms.
GeneSymbol	CREB1
PurificationMethod	ProteinAPurfied
UniProtNumber	P16220
UniProtSummary	FUNCTION:SwissProt:P16220#ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE. SIZE:341aminoacids;36688Da SUBUNIT:BindsDNAasadimer.InteractsPPRC1andwithHTLV-1Tax-1. SUBCELLULARLOCATION:Nucleus. PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPandEP300binding(Bysimilarity).PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P16220##BelongstothebZIPfamily.&Contains1bZIPdomain.&Contains1KID(kinase-inducIBLe)domain.
MolecularWeight	~43kDaobserved.

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Millipore/06-863 | Anti-CREB Antibody/06-863/200 µg

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