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Millipore/07-1231 | Anti-Notch1 Antibody, Intracellular/07-1231/200 µL

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貨號: 07-1231

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Description
CatalogueNumber	07-1231
Replaces	04-1046
Description	Anti-Notch1Antibody,Intracellular
AlternateNames	Neurogeniclocusnotchhomologprotein1precursor Notch(Drosophila)homolog1(translocation-associated) Notch1 Notchhomolog1,translocation-associated(Drosophila) Translocation-associatednotchproteinTAN-1 Neurogeniclocusnotchhomologprotein1
BackgroundInformation	Notchisafamilyofsingle-passtransmembranereceptorproteinsthataresynthesizedintheendoplasmicreticulumasaninactiveformandareproteolyticallycleavedonanextracellularsitebyafurin-likeconvertase(S1cleavage)inthetrans-golginetworkaftertherecognitionoftheRQRRsequencebeforeitreachestheplasmamembraneasheterodimerstoyieldanactive,ligand-accessIBLeform.TheNotchfamilyiscomprisedof4members(1-4)whoseligandsincludetheDeltaandJaggedfamilyofligands.TheseligandscauseproteolysisofNotchtoliberatetheintracellulardomain.CleavageresultsinaC-terminalfragmentN(TN)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleaved(S2cleavage)byTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcalledNotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilin-dependentgamma-secretasebetweengly1743andval1744(S3cleavage)toreleasetheintracellulardomain(NICD)fromthemembrane.ThatdomaintranslocatestothenucleusandinitiatestranscriptioneventsbybindingtheDNAbindingproteinCSL.Thenotchfamilymembersareinvolvedcelldifferentiationanddevelopment.

ProductInformation
Format	Serum
Control	HeLaCellLysate
Presentation	RabbitpolyclonalIgGseruminbuffercontainingin0.02MPotassiumphosphate,0.15MNaClwith0.01%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor6monthsat-20°Cinundilutedaliquotsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.

Applications
Application	UseAnti-Notch1Antibody,Intracellular(RabbitPolyclonalAntibody)validatedinELISA,WB,IHC(P)todetectNotch1alsoknownasNeurogeniclocusnotchhomologprotein1precursor.
KeyApplications	ELISA WesternBlotting Immunohistochemistry(Paraffin)
ApplicationNotes	ELISA:1:20,000-1:100,000dilutionfromapreviouslotwasusedinastandardsandwichELISAassayagainstthepeptideimmunogen. Optimalworkingdilutionsmustbedeterminedbytheenduser. Immunohistochemistry(paraffin): Representativetestingfromapreviouslot. OptimalStainingIGF-1WithEpitopeRetrieval:Lipoma.

BIOLOGicalInformation
Immunogen	SyntheticpeptidefromhumanNotch1.
Epitope	Intracellular
Host	Rabbit
Specificity	Notch1,intracellular.NoreactivityagainsthumanNotch2.
Isotype	IgG
SpeciesReactivity	Human
SpeciesReactivityNote	Human.NoreactivityobservedtomouseNotch.Reactivitywithotherspecieshasnotbeendetermined.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_017617.3
EntrezGeneSummary	ThisgeneencodesamemberoftheNotchfamily.MembersofthisType1transmembraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.Notchfamilymembersplayaroleinavarietyofdevelopmentalprocessesbycontrollingcellfatedecisions.TheNotchsignalingnetworkisanevolutionarilyconservedintercellularsignalingpathwaywhichregulatesinteractionsbetweenphysicallyadjacentcells.InDrosophilia,notchinteractionwithitscell-boundligands(delta,serrate)establishesanintercellularsignalingpathwaythatplaysakeyroleindevelopment.Homologuesofthenotch-ligandshavealsobeenidentifiedinhuman,butpreciseinteractionsbetweentheseligandsandthehumannotchhomologuesremaintobedetermined.Thisproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Thisproteinfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment.
GeneSymbol	NOTCH1 notch1 hN1 TAN1
UniProtNumber	P46531
UniProtSummary	FUNCTION:SwissProt:P46531#Functionsasareceptorformembrane-boundligandsJagged1,Jagged2andDelta1toregulatecell-fatedetermination.Uponligandactivationthroughthereleasednotchintracellulardomain(NICD)itformsatranscriptionalactivatorcomplexwithRBP-Jkappaandactivatesgenesoftheenhancerofsplitlocus.Affectstheimplementationofdifferentiation,proliferationandapoptoticprograms.Maybeimportantfornormallymphocytefunction.Inalteredform,maycontributetotransformationorprogressioninsomeT-cellneoplasms.InvolvedinthematurationofbothCD4+andCD8+cellsinthethymus.Maybeimportantforfolliculardifferentiationandpossiblycellfateselectionwithinthefollicle.Duringcerebellardevelopment,mayfunctionasareceptorforneuronalDNERandmaybeinvolvedinthedifferentiationofBergmannglia(Bysimilarity). SIZE:2556aminoacids;272500Da SUBUNIT:HeterodimerofaC-terminalfragmentN(TM)andanN-terminalfragmentN(EC)whichareprobablylinkedbydisulfidebonds.InteractswithDNER,DTX1,DTX2andRBPSUH.AlsointeractswithMAML1,MAML2andMAML3whichactastranscriptionalcoactivatorsforNOTCH1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein(Bysimilarity).&Notch1intracellulardomain:Nucleus(Bysimilarity).Note=FollowingproteolyticalprocessingNICDistranslocatedtothenucleus(Bysimilarity). TISSUESPECIFICITY:Infetaltissuesmostabundantinspleen,brainstemandlung.Alsopresentinmostadulttissueswhereitisfoundmainlyinlymphoidtissues. PTM:Synthesizedintheendoplasmicreticulumasaninactiveformwhichisproteolyticallycleavedbyafurin-likeconvertaseinthetrans-Golginetworkbeforeitreachestheplasmamembranetoyieldanactive,ligand-accessibleform.CleavageresultsinaC-terminalfragmentN(TM)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleavedbyTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcallednotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilindependentgamma-secretasetoreleaseanotch-derivedpeptidecontainingtheintracellulardomain(NICD)fromthemembrane(Bysimilarity).&Phosphorylated(Bysimilarity). DISEASE:SwissProt:P46531#NOTCH1truncationisassociatedwithT-cellacutelymphoblasticleukemia.&DefectsinNOTCH1areacauseofaorticvalvedisease[MIM:109730].Thedisorderconsistsofanearlydevelopmentaldefectintheaorticvalveandalaterde-repressionofcalciumdepositionthatcausesprogressiveaorticvalvedisease.CalcificationoftheaorticvalveisthethirdleADIngcauseofheartdiseaseinadults.Theincidenceincreaseswithage,anditisoftenassociatedwithabicUSPidaorticvalvepresentin1-2%ofthepopulation. SIMILARITY:SwissProt:P46531##BelongstotheNOTCHfamily.&Contains5ANKrepeats.&Contains36EGF-likedomains.&Contains3LNR(Lin/Notch)repeats.
MolecularWeight	~80kDa

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Millipore/07-1231 | Anti-Notch1 Antibody, Intracellular/07-1231/200 µL

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