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Millipore/07-1232 | Anti-Notch 1 Antibody, NT/07-1232/200 µL

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貨號: 07-1232

品牌: Millipore

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Description
CatalogueNumber	07-1232
Replaces	04-1046
Description	Anti-Notch1Antibody,NT
AlternateNames	Neurogeniclocusnotchhomologprotein1precursor Notch(Drosophila)homolog1(translocation-associated) Notch1 Notchhomolog1,translocation-associated(Drosophila) Translocation-associatednotchproteinTAN-1 Neurogeniclocusnotchhomologprotein1
BackgroundInformation	Notchisafamilyofsingle-passtransmembranereceptorproteinsthataresynthesizedintheendoplasmicreticulumasaninactiveformandareproteolyticallycleavedonanextracellularsitebyafurin-likeconvertase(S1cleavage)inthetrans-golginetworkaftertherecognitionoftheRQRRsequencebeforeitreachestheplasmamembraneasheterodimerstoyieldanactive,ligand-accessIBLeform.TheNotchfamilyiscomprisedof4members(1-4)whoseligandsincludetheDeltaandJaggedfamilyofligands.TheseligandscauseproteolysisofNotchtoliberatetheintracellulardomain.CleavageresultsinaC-terminalfragmentN(TN)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleaved(S2cleavage)byTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcalledNotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilin-dependentgamma-secretasebetweengly1743andval1744(S3cleavage)toreleasetheintracellulardomain(NICD)fromthemembrane.ThatdomaintranslocatestothenucleusandinitiatestranscriptioneventsbybindingtheDNAbindingproteinCSL.Thenotchfamilymembersareinvolvedcelldifferentiationanddevelopment.

ProductInformation
Format	Serum
Control	JurkateCellLysates.Infetaltissues,usespleen,brainstemandlung.Inadulttissuesusemainlylymphoidtissues.
Presentation	RabbitpolyclonalIgGseruminbuffercontaining0.02MPotassiumPhosphate,0.15MNaCl,pH7.2,containing0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor6monthsat-20°Cinundilutedaliquotsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.

Applications
Application	Anti-Notch1Antibody,N-TerminusisaRabbitPolyclonalAntibodyfordetectionofNotch1alsoknownasNeurogeniclocusnotchhomologprotein1precursor&hasbeenvalidatedinELISA,WB,IHC(P).
KeyApplications	ELISA WesternBlotting Immunohistochemistry(Paraffin)
ApplicationNotes	ELISA: Apreviouslotofthisantibodywasdilutedto1:20,000-1:60,000andusedinastandardsandwichELISAassayagainstthepeptideimmunogen. Immunohistochemistry(paraffin): Representativetestingfromapreviouslot. OptimalStainingofNotch-1WithCitratepH6.0EpitopeRetrieval:UterineCancer ELISA:Apreviouslotofthisantibodywasdilutedto1:20,000-1:60,000andusedinastandardsandwichELISAassayagainstthepeptideimmunogen.

BIOLOGicalInformation
Immunogen	SyntheticpeptidefromtheN-terminalsequenceofthecleavedNintracellulardomain(NICD)humanNotch1.
Epitope	CleavedN-Terminus
Host	Rabbit
Specificity	Notch1,cleavedNterminal.Onlythecleavedintracellular(activated)formisdetected.NoreactivityisdetectedagainstmouseN2,N3orN4.Thisepitopeisexposedonlyaftergammasecretasecleavageandisnotaccessibleintheuncleavedform.
Isotype	IgG
SpeciesReactivity	Human Mouse
SpeciesReactivityNote	Humanandmouse.Theantibodyisalsoexpectedtoreactwithratduetoimmunogensequencehomology.Otherspecieshavenotbeentested.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_017617.3
EntrezGeneSummary	ThisgeneencodesamemberoftheNotchfamily.MembersofthisType1transmembraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.Notchfamilymembersplayaroleinavarietyofdevelopmentalprocessesbycontrollingcellfatedecisions.TheNotchsignalingnetworkisanevolutionarilyconservedintercellularsignalingpathwaywhichregulatesinteractionsbetweenphysicallyadjacentcells.InDrosophilia,notchinteractionwithitscell-boundligands(delta,serrate)establishesanintercellularsignalingpathwaythatplaysakeyroleindevelopment.Homologuesofthenotch-ligandshavealsobeenidentifiedinhuman,butpreciseinteractionsbetweentheseligandsandthehumannotchhomologuesremaintobedetermined.Thisproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Thisproteinfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment.
GeneSymbol	NOTCH1 notch1 hN1 TAN1
UniProtNumber	P46531
UniProtSummary	FUNCTION:SwissProt:P46531#Functionsasareceptorformembrane-boundligandsJagged1,Jagged2andDelta1toregulatecell-fatedetermination.Uponligandactivationthroughthereleasednotchintracellulardomain(NICD)itformsatranscriptionalactivatorcomplexwithRBP-Jkappaandactivatesgenesoftheenhancerofsplitlocus.Affectstheimplementationofdifferentiation,proliferationandapoptoticprograms.Maybeimportantfornormallymphocytefunction.Inalteredform,maycontributetotransformationorprogressioninsomeT-cellneoplasms.InvolvedinthematurationofbothCD4+andCD8+cellsinthethymus.Maybeimportantforfolliculardifferentiationandpossiblycellfateselectionwithinthefollicle.Duringcerebellardevelopment,mayfunctionasareceptorforneuronalDNERandmaybeinvolvedinthedifferentiationofBergmannglia(Bysimilarity). SIZE:2556aminoacids;272500Da
MolecularWeight	~80kDa

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Millipore/07-1232 | Anti-Notch 1 Antibody, NT/07-1232/200 µL

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