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Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL

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貨號: 07-596

品牌: Millipore

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Description
CatalogueNumber	07-596
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-HistoneH4(citrulline3)Antibody
AlternateNames	Cit-H4R3 H4Cit3 HistoneH4,Arg3citrullinated
BackgroundInformation	HistoneH4(UniProtP62805;alsoknownasH4histonefamilymemberA,Histone1H4a)isencodedbytheHIST1H4A(alsoknownasH4/A,H4FA)gene(GeneID8359)inhuman.HistoneH4isoneofthemainhistoneproteinsthatcontributetothechromatinstructureinbothprokaryoticandeukaryoticcells.HistoneH4canundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses,includingmethylationandacetylationonlysineandarginineresidues,phosphorylationonserineandthreonineresidues,aswellasconversionArg3toacitrullineresiduebythepeptidylargininedeiminasePAD4-catalyzeddeiminationreaction,alsoknownascitrullination).HistoneH4Arg3citrullination(H4Cit3)weakenshistone-DNAinteractionasaresultofaconformationalchangeandareducedpositivechargeatthehistoneH4Nterminalend.BacterialinfectionisknowntostimulateH4Cit3inneutrophils,forminghighlydecondensedextracellularchromatinstructureNET(neutrophilextracellulartrap)thatcouldcaptureandkillmicroorganisms.Ontheotherhand,citrullinatedproteinsarerecognizedasnon-selfproteinsandsubsequentlyactivateimmunesystems.AfunctionalhaplotypeofthePADI4genehasbeenshowntobeassociatedwiththepathogenesisofrheumatoidarthritisthroughinductionoftheantigenicityofproteins.H4Cit3isinducedinresponsetoDNAdamagethroughthep53-PADI4pathway,resultinginupregulatedH4Cit3immunoreactivitylocalizedaroundfragmentednucleiinapoptoticcells.Inaddition,H4Cit3levelisnegativelycorrelatedwithp53proteinexpressionandtumoursizeinnon-smallcelllungcancer(NSCLC)tissues,indicatingacrucialroleofH4Cit3incarcinogenesis.

ProductInformation
Format	AffinityPurified
Presentation	Purifiedrabbitpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8°Cfromdateofreceipt.

Applications
Application	Anti-HistoneH4(Citrulline3)Antibody,Cat.No.07-596,isahighlyspecificrabbitpolyclonalantibodythattargetshistoneH4citrullination(H4Cit3)andhasbeentestedinChromatinImmunoprecipitation(ChIP),Immunocytochemistry,Immunohistochemistry,andWesternBlotting.
KeyApplications	ChromatinImmunoprecipitation(ChIP) Immunocytochemistry Immunohistochemistry(Paraffin) WesternBlotting
ApplicationNotes	ChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelot(3μLperChIP)detectedhistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsthathadbeentreatedwithβ-estradiolforonehouraftera3-dayserum-starvationperiod. ImmunocytochemistryAnalysis:A1:500dilutiondetectedfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullinationinionomycin-stimulatedprimaryhumanneutrophils(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics). ImmunohistochemistryAnalysis:A1:250dilutionfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics). ChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelotdetectedatime-dependenthistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsfollowingestradioltreatment(Wang,Y.,etal.(2004).Science.306(5694):279-283). ImmunocytochemistryAnalysis:ArepresentativelotdetectedenhancednuclearH4/H2AAr3citrullinationinHEK293TandU2OScellsuponexogenousPAD4expressionorDNAdamageinduction(Tanikawa,C.,etal.(2012).Nat.Commun.3:676). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeABIlizedcasein-elicitedperitonealneutrophilscellsfromwild-type,butnotPad4-knockoutmice(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeabilizedinfiltratinglungleukocytesisolatedfromwild-type,butnotPad4-knockoutmicefollowinginfluenzainfection(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in4%paraformaldehyde-fixed,1%NP-40/0.5%TritonX-100-permeabilizedperipheralbloodcellsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)insynovialcellsandextracellulartraps(NETs)flushedoutoftheanklejointsofwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104). ImmunocytochemistryAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4(H4Cit3)Arg3citrullinationin3.7%paraformaldehyde-fixed,0.2%NP-40/0.1%TritonX-100-permeabilizedHL-60cells(Wang,Y.,etal.(2004).Science.306(5694):279-283). ImmunohistochemistryAnalysis:Arepresentativelotdetectedgammairradiation-inducednuclearH4/H2AAr3citrullinationinthymustissuesectionsfromwild-type,butnotPad4-knockoutmice(Tanikawa,C.,etal.(2012).Nat.Commun.3:676). ImmunohistochemistryAnalysis:ArepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice10dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104). WesternBlottingAnalysis:ArepresentativelotdetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellasDNAdamage-inducedcellularH4andH2AAr3citrullination(Tanikawa,C.,etal.(2012).Nat.Commun.3:676). WesternBlottingAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4knockdownbyshRNAtreatmentgreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Wang,Y.,etal.(2004).Science.306(5694):279-283).

BIOLOGicalInformation
Immunogen	KLH-conjugated,syntheticpeptidecorrespondingtoaminoacids1-10(SG[cit]GKGGKGL-C)ofhumanhistoneH4(Met1removed),where[cit]denotescitrullinatedArg3.AC-terminalcysteinewasaddedforconjugationpurposes.
Epitope	N-terminus
Concentration	Pleaserefertolotspecificdatasheet.
Host	Rabbit
Specificity	ThisrabbitpolyclonalantibodydetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellascalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4(peptidylargininedeiminase4)knockdowngreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Tanikawa,C.,etal.(2012).Nat.Commun.3:676;Wang,Y.,etal.(2004).Science.306(5694):279-283).
SpeciesReactivity	Human Mouse
SpeciesReactivityNote	Human,Mouse.Predictedtoreactwithabroadrangeofspeciesbasedon100%sequencehomology.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_003529
GeneSymbol	HIST1H4A H4/A ;H4FA HIST1H4B H4/I ;H4FI HIST1H4C H4/G H4FG HIST1H4D H4/B H4FB HIST1H4E H4/J H4FJ HIST1H4F H4/C H4FC HIST1H4H H4/H H4FH HIST1H4I H4/M H4FM HIST1H4J H4/E H4FE HIST1H4K H4/D H4FD HIST1H4L H4/K H4FK HIST2H4A H4/N H4F2 H4FN HIST2H4 HIST2H4B H4/O H4FO HIST4H4 HIST1H4A H4/A
Modifications	Citrullination
PurificationMethod	Affinitypurified.
UniProtNumber	P62805
MolecularWeight	~12kDaobserved.11.24/11.37kDa(H4with/withoutMet1removal)calculated.Uncharacterizedbandsmaybeobservedinsomelysate(s).

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Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL

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