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Jackson/AMCA AffiniPure F(ab")? Fragment Goat Anti-Syrian Hamster IgG (H+L)/0.5 mg/107-156-142

價格: ￥1410.00 市場價: 3760.00

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貨號: 107-156-142

品牌: Jackson

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- Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule syrian hamster IgG.It also reacts with the light chains of other syrian hamster immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.
  F(ab")₂ fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab")₂ fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
  Coumarin AMCA
  Amax: 350Emax: 450nm
  Aminomethylcoumarin Acetate (AMCA) conjugates absorb light maximally around 350 nm and fluoresce maximally around 450 nm. For fluorescence microscopy, AMCA can be excited with a mercury lamp and observed using a UV filter set. Since blue fluorescence is not well detected by the human eye, AMCA-conjugated secondary antibodies should be used only with the most abundant antigens in multiple-labeling experiments. Ways of improving the visibility of AMCA include dark adapting the eyes, using fluorite instead of glass objectives, avoiding mounting media that absorb UV light (such as plastic-based media), and capturing photographic images with blue-sensitive film or CCD cameras. AMCA fades rapidly in conventional epifluorescence and confocal microscopy, and therefore it should be used with mounting media containing an anti-fading agent such as n-propyl gallate.
  Target: Syrian HamsterHost: GoatAntibody Format: F(ab')? Fragment Specificity: IgG (H+L)
  Minimal Cross Reactivity: Bovine, Horse, Human, Mouse, Rabbit, Rat Serum Proteins
  Conjugate: Coumarin AMCAProduct Category: F(ab')₂ Fragment Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2337463
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