Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG.It also reacts with the light chains of other human immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Biotin-SP (long spacer)
Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.
Minimal Cross Reactivity: Bovine, Horse, Mouse Serum Proteins
Conjugate: Biotin-SP (long spacer)Product Category: Whole IgG Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2337628
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