Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab")2/Fab portion of rat IgG.It also reacts with the light chains of other rat immunoglobulins.No antibody was detected against the Fc portion of rat IgG or against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with immunoglobulins from other species.
Horseradish Peroxidase
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Target: RatHost: GoatAntibody Format: Whole IgG Specificity: IgG, F(ab")? fragment specificMinimal Cross Reactivity: Human, Bovine, Horse Serum Proteins
Conjugate: Horseradish PeroxidaseProduct Category: Whole IgG Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2338136