Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG.It also reacts with the light chains of other rat immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with immunoglobulins from other species.
Alkaline Phosphatase
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.
Minimal Cross Reactivity: Human, Bovine, Horse Serum Proteins
Conjugate: Alkaline PhosphataseProduct Category: F(ab')₂ Fragment Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2338162
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