Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rat IgG and with those common to other rat immunoglobulins.Reaction is primarily with kappa light chains.The antibody does not react with the heavy chain of rat IgG.The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Biotin-SP (long spacer)
Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.
Minimal Cross Reactivity: Bovine, Goat, Horse, Human, Mouse, Rabbit, Sheep Immunoglobulins
Conjugate: Biotin-SP (long spacer)Product Category: Anti?Light Chain-Specific Antibodies for Western Blotting after IPClonality: Polyclonal RRID: AB_2338180
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