Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of rat IgM but not with rat IgG or the light chains of rat immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with IgM from other species.
Cyanine Cy?2
Amax: 492
Emax: 510nm
Cy2 conjugates have maximum absorption/excitation at 492 nm and fluoresce with a peak around 510 nm in the green region of the visible spectrum like FITC conjugates (520 nm), but they are more photostable and less sensitive to pH changes than FITC. However, for mounting in aqueous media we recommend Alexa Fluor? 488 as the preferred green-fluorescing dye because it is brighter and more photostable than Cy2, FITC, and DyLight 488. A further disadvantage of using Cy2 with aqueous mounting media is its sensitivity to p-phenylenediamine, an anti-fading agent found in some commercial mounting media, which results in weak and diffused fluorescence after storage of stained slides. The main advantage of Cy2 conjugates is increased fluorescence in plastic mounting media.
Minimal Cross Reactivity: Human, Bovine, Horse Serum Proteins
Conjugate: Cyanine Cy?2Product Category: Cyanine Conjugates for Permanent MountingClonality: Polyclonal RRID: AB_2338276
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