Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rat IgG and with those common to other rat immunoglobulins.Reaction is primarily with kappa light chains.The antibody does not react with the heavy chain of rat IgG.The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Alexa Fluor? 488
Amax: 493
Emax: 519nm
Alexa Fluor? 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor? 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.
Minimal Cross Reactivity: Bovine, Goat, Horse, Human, Mouse, Rabbit, Sheep Immunoglobulins
Conjugate: Alexa Fluor? 488Product Category: Anti?Light Chain-Specific Antibodies for Western Blotting after IPClonality: Polyclonal RRID: AB_2338363
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