Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins.No antibody was detected against rat IgM or non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with immunoglobulins from other species.
Brilliant Violet 480?
Amax: 436
Emax: 478nm
BD Brilliant™ Violet 480 (BV480) has an Excitation peak at 436 nm with maximal emission at 478 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor? 488 and Rhodamine Red™-X. If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor? 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.
Minimal Cross Reactivity: Human, Bovine, Horse Serum Proteins
Conjugate: Brilliant Violet 480?Product Category: Brilliant Violet™ Conjugates for Multiple LabelingClonality: Polyclonal RRID: AB_2651080
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