Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on mouse IgG and with those common to other mouse immunoglobulins.Reaction is primarily with kappa light chains.The antibody does not react with the heavy chain of mouse IgG.The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit,rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Alkaline Phosphatase
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.
Minimal Cross Reactivity: Bovine, Goat, Horse, Human, Rabbit,Rat, Sheep Immunoglobulins
Conjugate: Alkaline PhosphataseProduct Category: Anti?Light Chain-Specific Antibodies for Western Blotting after IPClonality: Polyclonal RRID: AB_2338541
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