Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2b but not with the Fab portion of mouse immunoglobulins.No antibody was detected against mouse IgM or againstnon-immunoglobulin serum proteins.The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.
DyLight? 405
Amax: 400
Emax: 421nm
DyLight 405-conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use is limited to microscopes or flow cytometers equipped with a 405 nm laser and a 420 nm emission filter. Under these conditions, it is possible to perform effective 4-color imaging with good color separation, good photostability, and high sensitivity. The combination of DyLight 405, Alexa Fluor? 488, Rhodamine Red-X, and Alexa Fluor? 647 provides for maximum color separation. Another 4-color dye combination, which may be equally effective but has slightly less color separation, is DyLight 405, Alexa Fluor? 488, Cy3, and Alexa Fluor? 647. DyLight 405 conjugates are an excellent choice for blue-fluorescing secondary antibodies in multi-color labeling protocols.