Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2a but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Brilliant Violet 421?
Amax: 407
Emax: 421nm
BD Brilliant™ Violet 421 (BV421) has an Excitation peak at 407 nm with maximal emission at 421 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
5-color imaging is possible with BV421, in combination with BV480, Alexa Fluor? 488, Rhodamine-Red™-X, and Alexa Fluor? 647. If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor? 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.
Minimal Cross Reactivity: Human, Bovine, Rabbit Serum Proteins
Conjugate: Brilliant Violet 421?Product Category: Brilliant Violet™ Conjugates for Multiple LabelingClonality: Polyclonal RRID: AB_2651089
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