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            當前位置 : Millipore >>> Millipore/12-439 | Retinoblastoma Protein (aa 773-928)/12-439/500 µg
            Millipore/12-439 | Retinoblastoma Protein (aa 773-928)/12-439/500 µg
            • Millipore/12-439 | Retinoblastoma Protein (aa 773-928)/12-439/500 µg

            Millipore/12-439 | Retinoblastoma Protein (aa 773-928)/12-439/500 µg

            價格: ¥3204.00 市場價: 5340.00

            貨號: 12-439
            品牌: Millipore
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              • Description
                CatalogueNumber12-439
                BrandFamilyUpstate
                TradeName
                • Upstate
                DescriptionRetinoblastomaProtein(aa773-928)
                OverviewRecombinantfusionproteincontainingtheC-terminalfragment(residues773-928)ofhumanretinoblastomaprotein(Rb)withanN-terminalHis6-tag,expressedinE.coli.
                ProductInformation
                Presentation50mMTris-HCl,pH7.5,150mMNaCl,and500mMimidazole
                StorageandShippingInformation
                StorageConditionsStablefor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.
                Applications
                ApplicationRecombinantfusionproteincontainingtheC-terminalfragment(residues773-928)ofhumanretinoblastomaprotein(Rb)withanN-terminalHis6-tag,expressedinE.coli.
                KeyApplications
                • KinaseAssay
                BIOLOGicalInformation
                Concentration2.0mg/mL
                EntrezGeneNumber
                EntrezGeneSummaryRetinoblastoma(RB)isanembryonicmalignantneoplasmofretinalorigin.Italmostalwayspresentsinearlychildhoodandisoftenbilateral.Spontaneousregression("cure")occursinsomecases.TheretinoblastomageneRBwasthefirsttumorsuppressorgenecloned,andisanegativeregulatorofthecellcyclethroughitsABIlitytobindthetranscriptionfactorE2F(MIM189971)andrepresstranscriptionofgenesrequiredforSphase(HanahanandWeinberg,2000[PubMed10647931]).[suppliedbyOMIM]
                GeneSymbol
                • RB1
                • RB
                • PP110
                • P105-RB
                • retinoblastoma-1
                • OSRC
                UniProtNumber
                UniProtSummaryFUNCTION:SwissProt:P06400#Keyregulatorofentryintocelldivisionthatactsasatumorsuppressor.Directlyinvolvedinheterochromatinformationbymaintainingoverallchromatinstructureand,inparticular,thatofconstitutiveheterochromatinbystabilizinghistonemethylation.RecruitsandtargetshistonemethyltransferasesSUV39H1,SUV420H1andSUV420H2,leADIngtoepigenetictranscriptionalrepression.ControlshistoneH4"Lys-20"trimethylation.AlsoactsasatranscriptionrepressorofE2Ftargetgenesbyrecruitingchromatin-modifyingenzymestopromoters.InhibitstheintrinsickinaseactivityofTAF1.FormsacomplexwithadenovirusE1AandwithSV40largeTantigen.MaybindandmodulatefunctionallycertaincellularproteinswithwhichTandE1Acompeteforpocketbinding.
                SIZE:928aminoacids;106159Da
                SUBUNIT:InteractspreferentiallywithtranscriptionfactorE2F1.TheunphosphorylatedforminteractswithARID3B,JARID1A,SUV39H1,MJD2A/JHDM3AandTHOC1.InteractswiththeN-terminaldomainofTAF1.InteractswithAATF,DNMT1,LIN9,LMNA,SUV420H1,SUV420H2,PELP1andTMPO-alpha.MayinteractwithKNTC2.InteractswithEID1andZUBR1.InteractswithARID4AandJARID1B.
                SUBCELLULARLOCATION:Nucleus.
                TISSUESPECIFICITY:Expressedintheretina.
                PTM:PhosphorylatedfromStoMphaseofthecellcycleandisdephosphorylatedinG1.T,butnotE1A,bindsonlytotheunphosphorylatedform.
                DISEASE:SwissProt:P06400#DefectsinRB1arethecauseofchildhoodcancerretinoblastoma(RB)[MIM:180200].RBisacongenitalmalignanttumorthatarisesfromthenuclearlayersoftheretina.Itoccursinabout1:20"000livebirthsandrepresentsabout2%ofchildhoodmalignancies.Itisbilateralinabout30%ofcases.AlthoughmostRBappearsporadically,about20%aretransmittedasanautosomaldominanttraitwithincompletepenetrance.Thediagnosisisusuallymadebeforetheageof2yearswhenstrabismusoragraytoyellowreflexfrompupil(cateye)isinvestigated.&DefectsinRB1areacauseofbladdercancer[MIM:109800].&DefectsinRB1areacauseofosteogenicsarcoma[MIM:259500].
                SIMILARITY:SwissProt:P06400##Belongstotheretinoblastomaprotein(RB)family.
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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