Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule alpaca IgG, and with llama IgG. The antibody reacts primarily with the Fc region, and is not recommended for detection of VHH antibodies. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Horseradish Peroxidase
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Target: AlpacaHost: GoatAntibody Format: Whole IgG Specificity: IgG (H+L)Minimal Cross Reactivity: Bovine, Human, Mouse, Rabbit, Rat Serum Proteins
Conjugate: Horseradish PeroxidaseProduct Category: Anti-Alpaca Secondary AntibodiesClonality: Polyclonal RRID: AB_2783751