Based on immunoelectrophoresis, Western blot and/or ELISA, the antibody reacts with the VHH domain of heavy chain (HC) alpaca IgG, subclasses 2 and 3; and with the VHH domain of llama IgG, subclasses 2 and 3.It also reacts with whole molecule alpaca and llama IgG1. No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with immunoglobulins from other species.
Alkaline Phosphatase
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.
Minimal Cross Reactivity: Bovine Serum Proteins
Conjugate: Alkaline PhosphataseProduct Category: Anti-Alpaca Secondary AntibodiesClonality: Polyclonal RRID: AB_2810911
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