Based on immunoelectrophoresis and/or ELISA, the antibody reacts with heavy chain (HC) alpaca IgG, subclasses 2 and 3. It also reacts with HC llama IgG, subclasses 2 and 3. No antibody was detected against alpaca or llama IgG1, light chains or non-immunoglobulin serum proteins. The antibody reacts primarily with the Fc region, and is not recommended for detection of VHH antibodies. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Alexa Fluor? 488
Amax: 493
Emax: 519nm
Alexa Fluor? 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor? 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.
Minimal Cross Reactivity: Bovine, Human, Mouse, Rabbit, Rat Serum Proteins
Conjugate: Alexa Fluor? 488Product Category: Anti-Alpaca Secondary AntibodiesClonality: Polyclonal RRID: AB_2783777
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