Content:5mgOverview
Stem
MACS?DAPTisasmallmoleculeinhibitorofgamma-secretasethatpreventsNotchsignaling.
Details
Backgroundinformation
StemMACS?DAPTisacell-permeabledipeptidethatinhibitstheproteaseγ-secretase,akeycomponentoftheNotchsignalingpathway.TheNotchpathwayisinvolvedinvariousdevelopmentalcellfateandlineagedecisions,makingthesmallmoleculeinhibitorDAPTavaluabletoolforthemodulationofstemcelldifferentiationincellculture.Inaddition,γ-secretaseplaysaroleinprocessingofamyloidprecursorprotein(APP)toβ-amyloid,acomponentofthesenileplaquescharacteristicforAlzheimer’sdisease.Inhibitionofγ-secretasebyDAPThasbeenshowntocauseadose-dependentreductionofβ-amyloidlevelsinprimaryneuronalculturesaswellasinvivo.
Applications
PublishedapplicationsforDAPTinclude:
- DifferentiationofhumanPSCsintoretinalpigmentepithelium(RPE)cells1
 - NeuraldifferentiationofhumanPSCs2
 - PancreaticdifferentiationofhumanPSCs3
 - NociceptordifferentiationfromhumanPSCs4