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            當前位置 : Millipore >>> Millipore/14-173 | MAP Kinase 2/Erk2 Protein, active, mouse, 10 μg/14-173/10 µgAlso available in 250 μg size --cal
            Millipore/14-173 | MAP Kinase 2/Erk2 Protein, active, mouse, 10 μg/14-173/10 µgAlso available in 250 μg size --cal
            • Millipore/14-173 | MAP Kinase 2/Erk2 Protein, active, mouse, 10 μg/14-173/10 µgAlso available in 250 μg size --cal

            Millipore/14-173 | MAP Kinase 2/Erk2 Protein, active, mouse, 10 μg/14-173/10 µgAlso available in 250 μg size --cal

            價格: ¥4812.00 市場價: 8020.00

            貨號: 14-173
            品牌: Millipore
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              • Description
                CatalogueNumber14-173
                BrandFamilyUpstate
                TradeName
                • Upstate
                DescriptionMAPKinase2/Erk2Protein,active,mouse,10μg
                OverviewN-terminalGST-tagged,recombinantMAPKinase2,expressedinE.colicells;activatedusingMEK1(Catalog#14-429).
                ProductInformation
                Presentation50mMTris-HCl,pH7.5,150mMNaCl,0.1mMEGTA,0.03%Brij-35,50%glycerol,1mMbenzamidine,0.2mMPMSF,0.1%2-mercaptoethanol.
                StorageandShippingInformation
                StorageConditionsStoreinundilutedaliquotsat-20°Cforuptooneyearfromthedateofreceipt.Avoidrepeatedefreeze-thawcycles.
                Applications
                ApplicationActive,N-terminalGST-tagged,recombinantMAPKinase2,expressedinE.colicells,activatedusingMEK1(Catalog#14-429).ForuseinKinaseAssays.
                KeyApplications
                • KinaseAssay
                BIOLOGicalInformation
                Sourcerecombinant,mouse,full-lengthMAPKinase2
                SpecificActivityForSpecificActivitydata,refertotheCertificateofAnalysisforindividuallotsofthisenzyme.
                EntrezGeneNumber
                EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals,andareinvolvedinawidevarietyofcellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.Theactivationofthiskinaserequiresitsphosphorylationbyupstreamkinases.Uponactivation,thiskinasetranslocatestothenucleusofthestimulatedcells,whereitphosphorylatesnucleartargets.Twoalternativelysplicedtranscriptvariantsencodingthesameprotein,butdifferingintheUTRs,havebeenreportedforthisgene.
                GeneSymbol
                • MAPK1
                • p40
                • PRKM2
                • MAPK2
                • PRKM1
                • p42-MAPK
                • p38
                • p41
                • ERK
                • ERK-2
                • ERT1
                • P42MAPK
                • p41mapk
                • ERK2
                ProteinTargetMAPK2
                PurificationMethodGlutathioneagarose;Ni2+/NTAagarose
                TargetSub-FamilyCMGC
                UniProtNumber
                UniProtSummaryFUNCTION:SwissProt:P63085#Serine/threoninekinasewhichactsasanessentialcomponentoftheMAPkinasesignaltransductionpathway.MAPK1/ERK2andMAPK3/ERK1arethe2MAPKswhichplayanimportantroleintheMAPK/ERKcascade.TheyparticipatealsoinasignalingcascadeinitiatedbyactivatedKITandKITLG/SCF.Dependingonthecellularcontext,theMAPK/ERKcascademediatesdiversebiologicalfunctionssuchascellgrowth,adhesion,survivalanddifferentiationthroughtheregulationoftranscription,translation,cytoskeletalrearrangements.ThetheMAPK/ERKcascadeplaysalsoaroleininitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactors.About160substrateshavealreadybeendiscoveredforERKs.Manyofthesesubstratesarelocalizedinthenucleus,andseemtoparticipateintheregulationoftranscriptionuponstimulation.However,othersubstratesarefoundinthecytosolaswellasinothercellularorganelles,andthoseareresponsIBLeforprocessessuchastranslation,mitosisandapoptosis.Moreover,theMAPK/ERKcascadeisalsoinvolvedintheregulationoftheendosomaldynamics,includinglysosomeprocessingandendosomecyclingthroughtheperinuclearrecyclingcompartment(PNRC);aswellasinthefragmentationoftheGolgiapparatusduringmitosis.
                COFACTOR:Magnesium(Bysimilarity).
                DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases.
                SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain.
                MolecularWeight67.8kDa
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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