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Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25

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貨號: 17-10047

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Description
CatalogueNumber	17-10047
BrandFamily	Upstate
TradeName	ChIPAb+ Upstate
Description	ChIPAb+HistoneH4-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
Overview	AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HistoneH4setincludestheHistoneH4antibody,anegativecontrolantibody(normalrabbitIgG),andqPCRprimerswhichamplifya110bpregionofhumanβ-Globin.TheHistoneH4andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH4-associatedchromatin.
AlternateNames	H4 HistoneH4 H4histonefamily,memberA histone1,H4a histonecluster1,H4a
BackgroundInformation	HistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.

ProductInformation
Format	Purified
Control	Includesnegativecontrolantibody(normalrabbitIgG)andprimersspecificforhumanβ-Globin.
Presentation	Anti-HistoneH4(rabbitmonoclonalIgG).Onevialcontaining50μLofproteinApurifiedrabbitmonoclonalIgGsupernatantinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidebeforeadditionofglycerolto40%.Storeat-20°C. NormalRabbitIgG.Onevialcontaining75μgofnormalrabbitIgGin75μLstoragebuffer.Storeat-20°C. ChIPPrimers,β-Globin.Onevialcontaining75μLof5μMofeachprimerspecificforhumanβ-Globin.Storeat-20°C. FOR:AGGACAGGTACGGCTGTCATC REV:TTTATGCCCAGCCCTGGCTC

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	ThisChIPAb+HistoneH4-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
KeyApplications	WesternBlotting ChromatinImmunoprecipitation(ChIP)
ApplicationNotes	ChromatinImmunoprecipitation: SonicatedchromatinpreparedfromHeLacells(1X10⁶cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusing2μLofeithernormalrabbitIgGor2μLofAnti-HistoneH4antibodyandtheMagnaChIP?AKit(Cat.#17-610). SuccessfulimmunoprecipitationoftotalHistoneH4associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,β-Globin,aswellasGAPDHpromoterControlPrimers,(Pleaseseefigures). DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated. PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails. WesternBlotAnalysis: RepresentativeLotData. HeLaAcidExtract(Lane1and3),HeLaAcidExtractfromsodiumbutyratetreatedcells(Lane2),HeLaAcidExtractfromcolcemidtreatedcells(Lane4)andrecombinanthistoneH4(Lane5)wereresolvedbyelectrophoresis,transferredtoPVDFmembranesandprobedwithAnti-HistoneH4(1:1,000dilution). ProteinswerevisualizedusingaDonkeyanti-rabbitIgGconjugatedtoHRPandvisualizedusingachemiluminescencedetectionsystem.(Pleaseseefigures).

BiologicalInformation
Immunogen	KLH-conjugatedsyntheticpeptidecorres-pondingtoaminoacids17-28ofHistoneH4.
Epitope	a.a25-28
Host	Rabbit
Specificity	RecognizesHistoneH4.
SpeciesReactivity	Human
SpeciesReactivityNote	Human.Widerangeofcross-reactivityexpectedbasedonsequencehomology.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_001029249
EntrezGeneSummary	HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Thisstructureconsistsofapproximately146bpofDNAwrappedaroundanucleosome,anoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.Thisgeneisfoundinahistoneclusteronchromosome1.Thisgeneisoneoffourhistonegenesintheclusterthatareduplicated;thisrecordrepresentsthetelomericcopy.[providedbyRefSeq].
GeneSymbol	HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH HIST1H4H H4F2 H4FM H4/A H4FK H4FG HIST2H4 H4FB H4/K HIST1H4I H4/N HIST1H4B H4FE H4/M H4/a HIST1H4E HIST4H4 HIST2H4A H4FC H4FI H4/H HIST1H4C H4/G HIST1H4K H4FJ H4FD H4/I H4/B H4/D H4/C HIST1H4J HIST1H4D H4/E
PurificationMethod	ProteinAPurfied
UniProtNumber	P62805
UniProtSummary	FUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:103aminoacids;11367Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins. SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
MolecularWeight	~11kDa

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Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25

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