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Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.

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貨號: 17-10048

品牌: Millipore

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Description
CatalogueNumber	17-10048
BrandFamily	Upstate
TradeName	ChIPAb+ Upstate
Description	ChIPAb+HistoneH2A.Z-ChIPValidatedAntibodyandPrimerSet
Overview	AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HistoneH2A.ZsetincludestheHistoneH2A.Zantibody,anegativecontrolantibody(normalsheepIgG),andqPCRprimerswhichamplifya166bpregionofhumanGAPDH.TheHistoneH2A.Zandnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2A.Z-associatedchromatin.
AlternateNames	H2Ahistonefamily,memberZ H2AZhistone
BackgroundInformation	H2A.Z,alsoknownasH2AFZ,isavariantofhistoneH2Avariantthatishighlyconservedfromyeasttohumans.Likeotherhistonevariants,H2A.Zisencodedbyauniquegene,resultinginaslightlydivergenttypeofH2Awithspecializedfunctions.H2A.ZisinvolvedinchromatincompactionbystABIlizingthehistoneoctamerwithinthenucleosome.Itpromotesgeneexpressionthroughthecreationofanopendomainofchromatinthatismoreeasilytranscribed.H2A.Zisnonrandomlydistributedthroughoutthegenome,targetedprimarilytopericentricheterochromatin,butexcludedfromtheinactiveXchromosomeandthenucleolus.H2A.Zisassociatedwithbotheuchromatinandfacultativeheterochromatin.DNAmethylationcaninfluencechromatinstructureandeffectgenesilencingbyexcludingH2A.Z,andlikewise,H2A.ZprotectsgenesfromDNAmethylation.Thus,H2A.Zplaysaroleinregulatingheterochromatinsilencingandisinvolvedintranscriptionalcontrol.

ProductInformation
Format	AffinityPurified
Control	IncludesnegativecontrolsheepIgGantibodyandprimersspecificforhumanGAPDH.
Presentation	Anti-HistoneH2A.Z(SheeppolyclonalIgG).Onevialcontaining25μgofaffinitypurifiedsheeppolyclonalIgGinPBS,pH7.4containing1%BSAwith0.02%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C. NormalSheepIgG.Onevialcontaining25μgofproteinGpurifiedsheepIgGin0.02Mpotassiumphosphate,0.15MNaCl,pH7.2,with0.01%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C. ControlPrimers.Onevialcontaining75μLof5μMofeachprimerspecificforhumanGAPDH.Storeat-20°C. FOR:TACTAGCGGTTTTACGGGCG REV:TCGAACAGGAGGAGCAGAGAGCGA

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	ThisChIPAb+HistoneH2A.Z-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
KeyApplications	ChromatinImmunoprecipitation(ChIP) WesternBlotting
ApplicationNotes	ChromatinImmunoprecipitation: SonicatedchromatinpreparedfromHeLacells(1X10⁶cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusing1μgofeitherNormalSheepIgGor1μgofAnti-HistoneH2A.ZandtheMagnaChIP?GKit(Cat.#17-611). SuccessfulimmunoprecipitationofHistoneH2A.ZassociatedDNAfragmentswasverifiedbyqPCRusingControlPrimers(PartNo.22-004)forapositive,andGAPDHCodingprimersasanegativeassay(Pleaseseefigures). DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated. PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails. WesternBlotAnalysis: Representativelotdata HeLalysatewasresolvedbyelectrophoresis,transferredtoPVDFandprobedwithanti-HistoneH2A.Z(1:1000dilution). Proteinswerevisualizedusingarabbitanti-sheepsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures).

BiologicalInformation
Immunogen	LinearpeptidecorrespondingtheC-terminalaminoacids113-127ofhumanHistoneH2A.Z.
Epitope	C-terminus
Host	Sheep
Specificity	RecognizesHistoneH2A.Z,Mr~15kDa.
SpeciesReactivity	Human
SpeciesReactivityNote	Human.Widespeciesreactivityisexpected. Predictedtocross-reactwithzebrafish,mouse,chicken,rat,rabbit,andXenopusbasedonsequencehomology.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_036544
EntrezGeneSummary	HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.Severaltranscriptvariantsencodingdifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq].
GeneSymbol	H2A.z H2A/z H2AZ MGC117173 H2AFZ
PurificationMethod	AffinityPurfied
UniProtNumber	P0C0S5
UniProtSummary	FUNCTION:VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Maybeinvolvedintheformationofconstitutiveheterochromatin.Mayberequiredforchromosomesegregationduringcelldivision.Ref.7 SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.H2AoritsvariantH2AFZformsanheterodimerwithH2B.H2AFZinteractswithINCENPBysimilarity. SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-122givesaspecifictagforepigenetictranscriptionalrepression. AcetylatedonLys-5,Lys-8andLys-12duringinterphase.AcetylationdisappearsatmitosisBysimilarity. NotphosphorylatedBysimilarity. SIMILARITY:BelongstothehistoneH2Afamily. MOLECULARWEIGHT:is13413.4Dafrompositions2-128.DeterminedbyESI.Monoisotopic,notmodified.Ref.8Ref.10Ref.11
MolecularWeight	~15kDa

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Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.

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