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Millipore/17-10246 | ChIPAb+? Histone H2A.Bbd - ChIP Validated Antibody and Primer Set/17-10246/25 assays25 assays per s

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貨號: 17-10246

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Description
CatalogueNumber	17-10246
TradeName	ChIPAb+ Upstate
Description	ChIPAb+?HistoneH2A.BBD-ChIPValidatedAntibodyandPrimerSet
Overview	AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HistoneH2A.BbdsetincludestheHistoneH2A.Bbdantibody,aNormalRabbitIgG,andcontrolprimerswhichamplifya70bpregionofChIPprimers,PGK1.TheHistoneH2A.Bbdandnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2A.Bbd-associatedchromatin.
AlternateNames	H2Ahistonefamily,memberB1 H2ABarrbody-deficient histoneH2A-Bbdtype1
BackgroundInformation	HistoneH2A.Bbd(Barrbody-deficient),alsoknownashistoneH2A-Bbdtype1,isanatypicalhistonevariant.ThishistonevariantassociateswithH2BandreplaceshistoneH2Ainthenucleosome.IncontrasttootherH2Ahistones,thisvariantlacksconservedresiduesthataretargetsofpost-translationalmodifications.ConsequentlythisH2A.Bbdvariantisabout15aminoacidsshorterandonly48%similartothecanonicalH2A.ThemajordifferencesbetweenthetwoarefoundinthedockingdomainthatlinksH2A-H2BtotheH3-H4tetramer.Nucleosomescontainingthishistoneadoptamorerelaxedstructureandprotect~118basepairsofDNAinsteadofthe147basepairsfoundinclassicalnucleosomes.ThisvarianthistoneisassociatedwithactiveXchromosomesandautosomes,andistypicallyabsentfrominactiveXchromosomes.

ProductInformation
Format	AffinityPurified
Control	IncludesnormalrabbitIgGandprimersspecificforhumanPGK1.
Presentation	Anti-HistoneH2A.Bbd(rabbitpolyclonal).Onevialcontaining100μgofpurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidebeforetheadditionof30%glycerol.Storeat-20°C Concentration:0.7mg/mL NormalRabbitIgG,.Onevialcontaining125μgofRabbitIgGin125μLofstoragebuffercontaining0.05%sodiumazide.Storeat-20°C. ChIPprimers,PGK1.Onevialcontaining75μLof5μMofeachprimerspecificforhumanPGK1.Storeat-20°C. FOR:CCTTCCCCCACGCTCCTA REV:TTGTGCAGGAACCTGTGAGATG

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	ThisChIPAb+HistoneH2A.Bbd-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
KeyApplications	ElectrophoreticMobilityShiftAssay Immunohistochemistry Immunoprecipitation WesternBlotting ChromatinImmunoprecipitation(ChIP)
ApplicationNotes	WesternBlotAnalysis: Representativelotdata. H2A.Bbdoverexpression(Lane1-2),variousrecombinantHistoneproteins(Lane4-7)andHeLaacidextract(Lane8)lysateswereprobedwithAnti-HistoneH2A.Bbd(0.5μg/mL); Lane1:500ngofH2A.Bbdoverexpressionlysate Lane2:1μgH2A.Bbdoverexpressionlysate Lane3:500ngVectortransfectedcelllysatecontrol Lane4:500ngRecombinantHistoneH2A(Cat.#:14-493) Lane5:500ngRecombinantHistoneH2B(Cat.#:14-491) Lane6:500ngRecombinantHistoneH3(Cat.#:14-494) Lane7:500ngRecombinantHistoneH4(Cat.#:14-697) Lane8:10μgHeLaacidextract ProteinswerevisualizedusingaDonkeyAntiRabbitIgGsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem. ArrowindicatesHistoneH2A.Bbd(~17kDa).(Figure2).

BiologicalInformation
Immunogen	KLH-conjugatedlinearpeptidecorrespondingtohumanHistoneH2A.Bbd.
Epitope	a.a.35-55
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	ThisantibodyrecognizeshumanHistoneH2A.Bbd.
SpeciesReactivity	Human Mouse
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_001017990
EntrezGeneSummary	HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.ThisgeneispartofaregionthatisrepeatedthreetimesonchromosomeX,onceinintron22oftheF8geneandtwiceclosertotheXqtelomere.Thisrecordrepresentsthemostcentromericcopywhichisinintron22oftheF8gene.[providedbyRefSeq].
GeneSymbol	H2A.Bbd H2AFB1
PurificationMethod	AffinityPurfied
UniProtNumber	P0C5Y9
UniProtSummary	FUNCTION:AtypicalhistoneH2AwhichcanreplaceconventionalH2Ainsomenucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Nucleosomescontainingthishistonearelessrigidandorganizeonly118basepairofDNAinsteadof147inclassicalnucleosomes.TheyareassociatedwithtranscriptionallyactivechromatinandexcludedformBarrbodies.Ref.4Ref.5 SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Maybeincorporatedintoaproportionofnucleosomes,replacingoneormoreH2Amolecules. SUBCELLULARLOCATION:Nucleus.Chromosome.Note:AssociatedwiththeactiveXchromosomeandwithautosomes,whileitisabsentfromtheinactiveXchromosomeandexcludedfromBarrbodies. DOMAIN:ThedockingdomainisresponsiblefortheweakerheterodimerizationwithH2B.Ref.4 MISCELLANEOUS:IncontrasttootherH2Ahistones,itdoesnotcontaintheconservedresiduesthatarethetargetofpost-translationalmodifications. SEQUENCESIMILARITIES:BelongstothehistoneH2Afamily.
MolecularWeight	~17kDaobserved

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