當前位置： Millipore >>> Millipore/17-461 | Phospho-EGFR (Tyr1173) STAR ELISA Kit/17-461/96 assays

Millipore/17-461 | Phospho-EGFR (Tyr1173) STAR ELISA Kit/17-461/96 assays

價格: 試用市場價: 0.00

打印產品信息

貨號: 17-461

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即申請

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	17-461
BrandFamily	Upstate
TradeName	STAR Upstate
Description	Phospho-EGFR(Tyr1173)STARELISAKit
AlternateNames	Epidermalgrowthfactorreceptor Proto-oncogenec-ErbB-1 Receptortyrosine-proteinkinaseerbB-1 EGFReceptor EGFR ERBB ERBB1
BackgroundInformation	I.TESTPRINCIPLE TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheEGFRplateiscoatedwithaspecificmousemonoclonalanti-EGFRcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingEGFRantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-EGFRantibodytodetectthecapturedphospho-EGFR(Tyr1173)ontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve. II.EGFRBACKGROUND Epidermalgrowthfactorreceptor(EGFR)isareceptortyrosinekinasethatplaysakeyroleintheregulationofessentialnormalcellularprocessesandinthepathophysiologyofhyperproliferativediseasessuchascancer.Epidermalgrowthfactorreceptor(EGFR)isareceptortyrosinekinaseknowntobeessentialformediationofbothproliferativeandsurvivalsignalstocells.ActivationoftheEGFRsignalingpathwayhasbeenlinkedwithincreasedcellproliferation,angiogenesis,metastasisanddecreasedapoptosis.EGFRisfoundinmostsolidtumorsanditsautophosphorylationactivatestheintrinsickinaseactivitytowardheterologoussubstrates,aswellascreatingdockingsitesforadapterproteinsformultipleadapterproteinstobindandnucleatesignalingcomplexesthatactivatetheRas,PI3Kinase,andPLCpathways. ManyoftheEGFRmutationsaredirectlyrelatedtovariousdiseasesandarethetargetofnumerousdrugs.Forinstance,theaminoacidsubstitutionL858RisoneofseveralheterozygousmutationsthathavebeenidentifiedinNon-Small-CellLungCancer(NSCLC)patientswhohaveclinicalresponsestotheEGFRinhibitorIressa?.ThereissomeevidencethatthesemutationsresultinelevatedactivityandenhancedsensitivitytoIressa?.InpatientswithtumorsbearingIressa?-sensitivemutations,resistantsubclonescontaininganadditionalEGFRmutation,T790M,emergeinthepresenceofthedrug.IthasbeenshownexperimentallythattheT790Mmutationleadstohigh-levelfunctionalresistancetoIressa.InpatientswithtumorsbearingIressa?-sensitivemutations(eg.L858R,L861Q),resistantsubclonescontainingtheT790Mmutationemergeinthepresenceofthedrug.
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000%micro;L 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater

ProductInformation
Components	1.CapturePlatepre-coatedwithanti-humanEGFRantibody:(PartNo.17-461A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch. 2.Anti-EGFR(Tyr1173)detectionantibody:(PartNo.17-461B)Onebottle(11mL)ofanti-EGFR(Tyr1173)detectionantibodycontainingsodiumazide,readytouse. 3.ELISADiluent:(PartNo.17-461C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse. 4.25XELISAWashBuffer:(PartNo.17-461D)Onebottle(50mL)of25XELISAWashBuffer. 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-461E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosol. 6.HRPDiluent:(PartNo.17-461F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol. 7.TMBSolution:(PartNo.17-461G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse. 8.StopSolution:(PartNo.17-461H)Onebottle(25mL)ofstopsolution,readytouse. 9.EGFR(Tyr1173)Standard:(PartNo.17-461I)TwovialsofEGFR(Tyr1173)standard,lyophilized. 10.PlateCovers:Twoplatecovers.
Detectionmethod	Chromogenic

ProductInformation

Components

1.CapturePlatepre-coatedwithanti-humanEGFRantibody:(PartNo.17-461A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
2.Anti-EGFR(Tyr1173)detectionantibody:(PartNo.17-461B)Onebottle(11mL)ofanti-EGFR(Tyr1173)detectionantibodycontainingsodiumazide,readytouse.
3.ELISADiluent:(PartNo.17-461C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
4.25XELISAWashBuffer:(PartNo.17-461D)Onebottle(50mL)of25XELISAWashBuffer.
5.Anti-RabbitIgGHRPconjugate:(PartNo.17-461E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosol.
6.HRPDiluent:(PartNo.17-461F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol.
7.TMBSolution:(PartNo.17-461G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
8.StopSolution:(PartNo.17-461H)Onebottle(25mL)ofstopsolution,readytouse.
9.EGFR(Tyr1173)Standard:(PartNo.17-461I)TwovialsofEGFR(Tyr1173)standard,lyophilized.
10.PlateCovers:Twoplatecovers.

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	1yearat4°C

Applications
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human Mouse Rat
AnalytesAvailable	EGFR
EntrezGeneNumber	NM_005228.3 NM_201282.1 NM_201283.1 NM_201284.1
GeneSymbol	EGFR ERBB1 ERBB mENA
Modifications	Phosphorylation
UniProtNumber	P00533
UniProtSummary	FUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST. SIZE:1210aminoacids;134277Da SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted. TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers. PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain. MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation.

PhysicochemicalInformation
Sensitivity	Sensitivity:1unit/mL. RangeofDetection:1.6to100units/mL

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Parafilm M封口膜/PM996/4x125/盒
￥0.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥850.00
ThermoFisherQ32856Qubit? Assay Tubes現貨
￥590.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00

Millipore/17-461 | Phospho-EGFR (Tyr1173) STAR ELISA Kit/17-461/96 assays

客戶服務

何為188

關注188微信公眾號