當前位置： Millipore >>> Millipore/17-469 | Met STAR ELISA Kit/17-469/96 assays

Millipore/17-469 | Met STAR ELISA Kit/17-469/96 assays

價格: 試用市場價: 0.00

打印產品信息

貨號: 17-469

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即申請

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	17-469
BrandFamily	Upstate
TradeName	STAR Upstate
Description	MetSTARELISAKit
BackgroundInformation	TestPrinciple TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheMetplateiscoatedwithaspecificmousemonoclonalanti-Metcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingMetantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-MetantibodytodetectthecapturedMetontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve. MetBackground MetisatyrosinekinasereceptorforHepatocyteGrowthFactor(HGF),thoughttostimulatemultiplecellularprocessesincludingcellproliferation,differentiation,cellmigrationandtumOrigenesis.ChronicstimulationofMetoncancercellsisthoughttoplayaroleinmetastasis.
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000μL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater

ProductInformation
Components	1.CapturePlatepre-coatedwithanti-humanMetantibody:(PartNo.17-469A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch. 2.Anti-Metdetectionantibody:(PartNo.17-469B)Onebottle(11mL)ofanti-Metdetectionantibodycontainingsodiumazide,readytouse. 3.ELISADiluent:(PartNo.17-469C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse. 4.25XELISAWashBuffer:(PartNo.17-469D)Onebottle(50mL)of25XELISAWashBuffer. 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-469E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosol. 6.HRPDiluent:(PartNo.17-469F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol. 7.TMBSolution:(PartNo.17-469G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse. 8.StopSolution:(PartNo.17-469H)Onebottle(25mL)ofstopsolution,readytouse. 9.MetStandard:(PartNo.17-469I)TwovialsofMetstandard,lyophilized. 10.PlateCovers:Twoplatecovers.
Detectionmethod	Chromogenic

ProductInformation

Components

1.CapturePlatepre-coatedwithanti-humanMetantibody:(PartNo.17-469A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
2.Anti-Metdetectionantibody:(PartNo.17-469B)Onebottle(11mL)ofanti-Metdetectionantibodycontainingsodiumazide,readytouse.
3.ELISADiluent:(PartNo.17-469C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
4.25XELISAWashBuffer:(PartNo.17-469D)Onebottle(50mL)of25XELISAWashBuffer.
5.Anti-RabbitIgGHRPconjugate:(PartNo.17-469E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosol.
6.HRPDiluent:(PartNo.17-469F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol.
7.TMBSolution:(PartNo.17-469G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
8.StopSolution:(PartNo.17-469H)Onebottle(25mL)ofstopsolution,readytouse.
9.MetStandard:(PartNo.17-469I)TwovialsofMetstandard,lyophilized.
10.PlateCovers:Twoplatecovers.

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	1yearat4°C

Applications
Application	TheMetcolorimetricSTARELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtoquantifyspecificlevelsofsignalingtargetsindenaturedcellextracts.
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	Met
EntrezGeneNumber	NM_000245.2
EntrezGeneSummary	Theproto-oncogeneMETproductisthehepatocytegrowthfactorreceptorandencodestyrosine-kinaseactivity.Theprimarysinglechainprecursorproteinispost-translationallycleavedtoproducethealphaandbetasubunits,whicharedisulfidelinkedtoformthematurereceptor.VariousmutationsintheMETgeneareassociatedwithpapillaryrenalcarcinoma.
GeneSymbol	MET HGFR AUTS9 c-Met RCCP2
UniProtNumber	P08581
UniProtSummary	FUNCTION:SwissProt:P08581#Receptorforhepatocytegrowthfactorandscatterfactor.Hasatyrosine-proteinkinaseactivity. SIZE:1390aminoacids;155527Da SUBUNIT:Heterodimerformedofanalphachain(50kDa)andabetachain(145kDa)whicharedisulfidelinked.BindsPLXNB1andGRB2.InteractswithSPSB1,SPSB2andSPSB4(Bysimilarity).InteractswithINPP5D/SHIP1.WhenphosphorylatedatTyr-1356,interactswithINPPL1/SHIP2.InteractswithRANBP9andRANBP10. SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. DISEASE:SwissProt:P08581#ActivationofMETafterrearrangementwiththeTPRgeneproducesanoncogenicprotein.&DefectsinMETmaybeassociatedwithgastriccancer.&DefectsinMETareacauseofhepatocellularcarcinoma(HCC)[MIM:114550].&DefectsinMETareacauseofhereditarypapillaryrenalcarcinoma(HPRC)[MIM:605074];alsoknownaspapillaryrenalcellcarcinoma2(RCCP2).HPRCisaformofinheritedkidneycancercharacterizedbyapredispositiontodevelopmultiple,bilateralpapillaryrenaltumors.Thepatternofinheritanceisconsistentwithautosomaldominanttransmissionwithreducedpenetrance.&GeneticvariationsinMETmaybeassociatedwithsusceptibilitytoautismtype1B(AUTS1B)[MIM:608636].Autismisaneurodevelopmentaldisordercharacterizedbydisturbanceinlanguage,perceptionandsocialization.Thedisorderisclassicallydefinedbyatriadoflimitedorabsentverbalcommunication,alackofreciprocalsocialinteractionorresponsiveness,andrestricted,stereotypical,andritualizedpatternsofinterestsandbehavior. SIMILARITY:SwissProt:P08581##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.&Contains3IPT/TIGdomains.&Contains1proteinkinasedomain.&Contains1Semadomain.

PhysicochemicalInformation
Sensitivity	Sensitivity:0.4ng/mL. RangeofDetection:0.4to25ng/mL

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Parafilm M封口膜/PM996/4x125/盒
￥0.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥850.00
ThermoFisherQ32856Qubit? Assay Tubes現貨
￥590.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00

Millipore/17-469 | Met STAR ELISA Kit/17-469/96 assays

客戶服務

何為188

關注188微信公眾號