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Millipore/17-630 | ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set/17-630/25 assays25 assays per set.

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貨號: 17-630

品牌: Millipore

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Description
CatalogueNumber	17-630
BrandFamily	Upstate
TradeName	ChIPAb+ Upstate
Description	ChIPAb+Acetyl-HistoneH4-ChIPValidatedAntibodyandPrimerSet
Overview	AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+Acetyl-HistoneH4setincludespurifiedrabbitpolyclonalantiserumandthenormalrabbitIgGantiserum,whichcanbeusedtodemonstratethattheacetyl-histoneH4antibodyiscapableofprecipitatingacetyl-histoneH4associatedchromatin.TheqPCRprimersincludedflankthehumanGAPDHpromoterandproducea166basepairPCRproduct.

ProductInformation
Format	Serum
Control	IncludednegativecontrolrabbitIgGantiserumandcontrolprimersspecificforhumanGAPDHpromoter.
Presentation	Anti-Acetyl-HistoneH4(rabbitpolyclonalIgG).Onevialcontaining100μLofantiserumcontaining0.05%sodiumazide.Storeat-20°C. NormalRabbitSerum.Onevialcontaining100uLantiserumcontaining0.05%sodiumazide.Storeat-20°C. ControlPrimers.Onevialcontaining75μLof5μMofeachprimerspecificforforhumanGAPDH.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt.

Applications
Application	ThisChIPAb+Acetyl-HistoneH4-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
KeyApplications	WesternBlotting Multiplexing CellFunctionAssay ChromatinImmunoprecipitation(ChIP)
ApplicationNotes	WesternBlotAnalysis: AcidextractedproteinsfromnormalHeLacells(Lane1)andHeLacellstreatedwith5mMsodiumbutyratefor24hours(Lane2)wereresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-acetylHistoneH4(1:2000). Proteinswerevisualizedusingagoat-antirabbitsecondaryantibodyconjugatedtoHRPandachemi-luminescencedetectionsystem.(Pleaseseefigures).

BiologicalInformation
Immunogen	Theacetyl-histoneH4antiserumismadeagainstapeptidecorrespondingtoaminoacids2-19ofTetrahymenahistoneH4.
Host	Rabbit
Specificity	RecognizesacetylatedhistoneH4ofapproximately10kDa.Cross-reactswithacetylatedhistoneH2BfromTetrahymenaandweaklycrossreactswithacetylatedhistoneH2BfromHeLacells. Maycross-reactwithotheracetylatedproteins.
SpeciesReactivity	Human Eukaryote
SpeciesReactivityNote	Proventoreactwithhumansamples.Predictedbroadcross-reactivityamongeukaryotesbaseduponsequencesimilarity.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_778224
UniProtNumber	P62805
UniProtSummary	Function:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. Subunitstructure:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. Subcellularlocation:Nucleus. Post-translationalmodification:AcetylationatLys-6(H4K5ac),Lys-9(H4K8ac),Lys-13(H4K12ac)andLys-17(H4K16ac)occursincodingregionsofthegenomebutnotinheterochromatin. CitrullinationatArg-4(H4R3ci)byPADI4impairsmethylation. MonomethylationatArg-4(H4R3me1)byPRMT1favorsacetylationatLys-9(H4K8ac)andLys-13(H4K12ac).DemethylationisperformedbyJMJD6. SymmetricdimethylationonArg-4(H4R3me2a)bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage. Monomethylated,dimethylatedortrimethylatedatLys-21(H4K20me1,H4K20me2,H4K20me3).MonomethylationisperformedbySET8.TrimethylationisperformedbySUV420H1andSUV420H2andinducesgenesilencing. UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirradiation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.MonoubiquitinatedatLys-92ofhistoneH4(H4K91ub1)inresponsetoDNAdamage.TheexactroleofH4K91ub1inDNAdamageresponseisstillunclearbutitmayfunctionasalicensingsignalforadditionalhistoneH4post-translationalmodificationssuchasH4Lys-21methylation(H4K20me). Sumoylated,whichisassociatedwithtranscriptionalrepression. Sequencesimilarities:BelongstothehistoneH4family.
MolecularWeight	10kDa

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Millipore/17-630 | ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set/17-630/25 assays25 assays per set.

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