IgG fraction Monoclonal Mouse Anti-Fluorescein may be used either as direct conjugates, or for more sensitivity, they can be used unconjugated followed by a conjugated anti-mouse IgG (H+L) for signal enhancement.
Alexa Fluor? 594
Amax: 591
Emax: 614nm
Alexa Fluor? 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Alexa Fluor? 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.
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