Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rabbit IgG and with those common to other rabbit immunoglobulins.The antibody does not react with the heavy chain of rabbit IgG.The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, armenian hamster, horse, human, mouse,rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Horseradish Peroxidase
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Minimal Cross Reactivity: Bovine, Goat, Armenian Hamster, Horse, Human, Mouse,Rat, Sheep Immunoglobulins
Conjugate: Horseradish PeroxidaseProduct Category: Anti?Light Chain-Specific Antibodies for Western Blotting after IPClonality: Monoclonal RRID: AB_2339149
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