Based on immunoelectrophoresis and/or ELISA, the antibody reacts with both human IgG and IgM.It also reacts with the light chains of other human immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Coumarin AMCA
Amax: 350
Emax: 450nm
Aminomethylcoumarin Acetate (AMCA) conjugates absorb light maximally around 350 nm and fluoresce maximally around 450 nm. For fluorescence microscopy, AMCA can be excited with a mercury lamp and observed using a UV filter set. Since blue fluorescence is not well detected by the human eye, AMCA-conjugated secondary antibodies should be used only with the most abundant antigens in multiple-labeling experiments. Ways of improving the visibility of AMCA include dark adapting the eyes, using fluorite instead of glass objectives, avoiding mounting media that absorb UV light (such as plastic-based media), and capturing photographic images with blue-sensitive film or CCD cameras. AMCA fades rapidly in conventional epifluorescence and confocal microscopy, and therefore it should be used with mounting media containing an anti-fading agent such as n-propyl gallate.
Target: HumanHost: RabbitAntibody Format: Whole IgG Specificity: IgG + IgM (H+L)Minimal Cross Reactivity: Mouse Serum Proteins
Conjugate: Coumarin AMCAProduct Category: Whole IgG Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2339705