Cyanine5amineisareactivedyewhichcontainsaminogroup,ananalogofCy5®amine.Thisreagentcanbecoupledwithavarietyofactivatedestersandotherelectrophilicreagents.Forexample,thisaminecanbecoupledwithEDC-activatedcarboxylicgroups.
Thisbrightandphotostabledyeissuitableformanydifferentmethodsoffluorescencedetection.Colorfulfluorophorecanalsobeeasilydetectedinsmallquantities(nanomols)bynakedhumaneye.
Generalproperties
| Appearance: | darkbluepowder |
| Molecularweight: | 653.77 |
| Molecularformula: | C38H54Cl2N4O |
| Solubility: | moderatesolubilityinwater,goodinpolarorganicsolvents(DMF,DMSO,alcohols) |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight. |
Spectralproperties
| Excitationmaximum,nm: | 646 |
| ε,L⋅mol−1⋅cm−1: | 250000 |
| Emissionmaximum,nm: | 662 |
| Fluorescencequantumyield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |