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Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/

價格: ￥4440.00 市場價: 7400.00

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貨號: 506123

品牌: Millipore

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Description
Overview	Recognizesthe~38kDap38MAPKprotein. AntibodyTargetGeneSymbol:MAPK14 TargetSynonym:CRK1,CSBP,CSBP1,CSBP2,CSPB1,EXIP,Hog,MAPKp38,MGC102436,MGC105413,MXI2,P38,P38KINASE,P38MapKinase,p38Mapkalpha,P38-ALPHA,p38-RK,p38/Hog1,p38/Mpk2,P38/RK,p38a,p38Hog,p38MAPK,PRKM14,PRKM15,RK,SAPK2A EntrezGeneName:mitogen-activatedproteinkinase14 HuEntrezID:1432 MuEntrezID:26416 RatEntrezID:81649
CatalogueNumber	506123
BrandFamily	Calbiochem®
ApplicationData	Detectionofp38MAPkinasebyimmunoblotting.Samples:C6cells(lanes1and2)treated(lane1)oruntreated(lane2)withanisomycinandNIH/3T3cells(lanes3and4)treated(lane3)oruntreated(lane4)withUV.Primaryantibody:Anti-p38MAPKinase(341-360)RabbitpAb(Cat.No.506123)(1:1000).Detection:chemiluminescence.

ProductInformation
Form	Liquid
Formulation	In150mMNaCl,10mMHEPES,50%glycerol,0.01%BSA,pH7.5.
Preservative	None

StorageandShippingInformation
ShipCode	BlueIceOnly
Toxicity	StandardHandling
Storage	-20°C
Avoidfreeze/thaw	Avoidfreeze/thaw
Donotfreeze	Oktofreeze
SpecialInstructions	Followinginitialthaw,aliquotandfreeze(-20°C).

Applications
KeyApplications	FlowCytometry Immunoblotting(WesternBlotting) ParaffinSections
ApplicationNotes	FlowCytometry(1:25) Immunoblotting(1:1000) ParaffinSections(1:50,heatpretreatmentrequired,seecomments)
ApplicationComments	Pretreatparaffinsectionsbyheatingtissuein10mMcitratebuffer,pH6.0for1minathighpowerfollowedby9minatmediumpower;keeptheslidesfullyimmersedandmaintainthetemperatureatorjustbelowboiling;cooltheslidesfor20minatroomtemperaturepriortostaining.Variablesassociatedwithassayconditionswilldictatetheproperworkingdilution. RecommendedProtocolforImmunoblotting SolutionsandReagents ?TransferBuffer:25mMTrisbase,0.2Mglycine,20%methanol,pH8.5. ?SDSSampleBuffer:62.5mMTris-HCl,pH6.8,2%SDS,10%glycerol,50mMDTT,0.1%bromophenolblue. ?10XTBS(Tris-bufferedsaline):Toprepare1liter,24.2gTrisbase,80gNaCl,adjustpHto7.6withHCl.Dilute1:10foruse. ?BlockingBuffer:1XTBS,0.1%Tween^?-20detergentwith5%non-fatdrymilk. ?PrimaryAntibodyDilutionBuffer:1XTBS,0.1%Tween^?-20detergentwith5%BSA ?WashBuffer(TBST):1XTBS,0.1%Tween^?-20detergent BlottingMembrane NitrocelluloseorPVDFmembranesmaybeused. ProteinBlotting 1.Lysecellsbyadding100mlSDSSampleBufferandimmediatelyscrapethecellsofftheplateandtransfertheextracttoamicrofugetube.Keeponice. 2.Sonicatefor2stoshearDNAandreducesampleviscosity. 3.Heatsampleto95-100°Cfor5min.Coolonice. 4.Microcentrifugefor5min. 5.Load20mlontoSDS-PAGEgel(10cmx10cm). 6.Electrotransfertonitrocellulosemembrane. Ascontrols,werecommendusing15mlofphosphorylatedandnonphosphorylatedC-6gliomacellextracts. MembraneBlocking,GelandAntibodyIncubations 1.Aftertransfer,washmembranewith25mlTBSfor5minatroomtemperature. 2.Incubatemembranein25mlofBlockingBufferfor1-3hatroomtemperatureorovernightat4°C. 3.Wash3timesfor5mineachwith15mlTBST. 4.Incubatemembraneandprimaryantibody(attheappropriatedilution)in10mlPrimaryAntibodyDilutionBufferwithgentleagitationovernightat4°C. 5.Wash3timesfor5mineachwith15mlTBST. 6.Incubatemembranewithconjugatedsecondaryantibodyattheappropriatedilutionin10mlBlockingBufferwithgentleagitationfor1hatroomtemperature. 7.Washmembraneasinstep5. DetectionofProteins Chemiluminescence.

BIOLOGicalInformation
Immunogen	asyntheticpeptide(TYDEYISFVPPPLDQEEMES)correspondingtoaminoacids341-360ofhumanp38MAPkinase
Immunogen	Human
Host	Rabbit
Isotype	IgG
SpeciesReactivity	Human Mouse Rat
AntibodyType	PolyclonalAntibody

PhysicochemicalInformation

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MaterialsInformation

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Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/

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