Properties:
| Related Categories |
Analytical/Chromatography,
Ascentis Express Columns for Fast HPLC,
Ascentis Express F5, 2μm UHPLC Columns,
F5, 2μm Columns,
HPLC,
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feature
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endcapped
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parameter
|
60 °C temp. range
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L × I.D.
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5 cm × 3 mm
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impurities
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<5 ppm metals
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matrix
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Fused-Core particle platform
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matrix active group
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PFP (pentafluorophenyl) phase
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particle size
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2 μm
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pore size
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90 ? pore size
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operating pH
|
2-9
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mode of chromatography
|
Reversed phase
|
Description:
General description
Ascentis Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core® particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4-micron thick porous shell and the small overall particle size of 2-microns. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5phases also retain compounds by polar interactions. Having polarr and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
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Legal Information
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.