Tetrazine-cyclooctene(TCO)ligationisoneofthefastestbioconjugationreactionsknowntodate.Inordertouseitforfluorescentlabeling,afluorescentreportermoleculebearingoneofthereactinggroupsisnecessary.
Cyanine5tetrazineisamoleculebearingCyanine5fluorophore,andtetrazinemoiety.Cyanine5isaverypopularfluorophorecompatIBLewithnumerousinstruments.
Generalproperties
| Appearance: | darkbluesolid |
| MassspecM+increment: | 637.4 |
| Molecularweight: | 811.84 |
| Molecularformula: | C42H48N7F6OP |
| Solubility: | goodinDMF,DMSO,DCM |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
| Excitationmaximum,nm: | 646 |
| ε,L⋅mol−1⋅cm−1: | 250000 |
| Emissionmaximum,nm: | 662 |
| Fluorescencequantumyield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |