Properties:
| RelatedCategories | AnalyticalColumn,3μm,Analytical/Chromatography,DiscoveryHPLCColumns,DiscoveryHSF5,HPLC, | 
| feature | endcapped | 
| extentoflabeling | 12%CarbonloADIng | 
| parameter | ≤70°Ctemp.range | 
| application(s) | HPLC:suitable | 
| L×I.D. | 10 cm×2.1 mm | 
| surfacecoverage | 4 μmol/m2 | 
| impurities | <10 ppmmetals | 
| matrix | silicagel,highpurity,sphericalparticleplatform | 
| matrixactivegroup | PFP(pentafluorophenyl)phase | 
| particlesize | 3 μm | 
| poresize | 120 ? | 
| surfacearea | 300 m2/g | 
| operatingpHrange | 2-8 | 
| suitABIlity | suitableforL43perUSP | 
| modeofchromatography | Hydrophilicinteraction(HILIC) | 
|  | Reversedphase | 
Description:
FeaturesandBenefits
• Uniqueselectivity
• SimilarretentiontoC18(sometimesrequiresstrongermobilephase)
• Excellentpeakshape
• Stable,low-bleedLC-MSseparations
• Scalableseparationsfrom3to10μmparticlesizes
Generaldescription
GuidelinesfortransferringaC18methodtoDiscovery®HSF5:
Generally,basesareretainedlongerontheHSF5thanonaC18.IncreasingtheorganiccontentofaC18separation5to10percentwillgenerallyprovidesimilarretentiononanHSF5.Resultswithothercompoundsarehighlyvariable.However,itisgenerallytruethatsoluteswithlog?Po/wvalueslessthan2.5willberetainedlongeronHSF5comparedtoaC18.Thedegreeofdifferenceishighlysolutedependent.
TheDiscovery®HSF5bondedphaseprovidesreversed-phaseseparationsthataredistinctlydifferentfromC18columns.However,compoundswillgenerallyelutewithinthesameretentiontimewindow,makingmostC18methodseasilytransferable.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
LegalInformation
DiscoveryisaregisteredtrademarkofSigma-AldrichCo.LLC