Synonym:
Hisep™ 5UM 5CMX4.6MM HPLC COLUMN
Properties:
| Related Categories |
Analytical Column, 5 Micron,
Analytical/Chromatography,
Direct Serum Injection,
HPLC,
HPLC Columns, by Brand for Small Molecules,
|
|
parameter
|
≤70 °C temp. range
|
|
L × I.D.
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5 cm × 4.6 mm
|
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matrix
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silica gel, spherical particle platform
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particle size
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5 μm
|
|
pore size
|
120 ?
|
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compatibility
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mode of use direct injection (shielded hydrophobic HPLC
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mode of chromatography
|
Mixed mode
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Description:
General description
The Hisep column uses a shielded hydrophobic phase (SHP) for its mechanism of separation. The silica-based material is covered with a thin polymer consisting of hydrophobic regions in a hydrophilic network. Small analytes, such as pharmaceuticals, penetrate the hydrophilic network and are retained by the hydrophobic moieties. The hydrophilic network shields protein molecules from contact with the surface and the hydrophobic groups, and thus these molecules are not retained. Direct injection of biological samples eliminates time-consuming cleanup steps and increases analytical accuracy.
Excellent column stability and an ability to exclude proteins over a wide pH range set Hisep columns apart from other direct serum injection columns and techniques. Stability is demonstrated in Figure A; no significant change is seen in the chromatography of trimethoprim after injecting 10mL of serum, in 10 μL increments, onto a Hisep column. A low pH application is shown in Figure B.
Not recommended for LC-MS analyses.
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Legal Information
Hisep is a trademark of Sigma-Aldrich Co. LLC
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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