Cyanine3hydrazideisacarbonyl-reactivedye,ananalogofCy3®hydrazide.
Thisreagentallowstolabelvariouscarbonyl-containingmoleculessuchasantibodiesandotherglycoproteinsafterperiodateoxidation,proteinswhichhaveundergoneoxidativestressordeamination,orreducingsaccarides.Cyanine3iscompatIBLewithanumberoffluorescentinstruments.
Generalproperties
| Appearance: | redpowder |
| Molecularweight: | 543.57 |
| Molecularformula: | C30H40Cl2N4O |
| Solubility: | moderatesolubilityinwater,goodinpolarorganicsolvents(DMF,DMSO,alcohols) |
| Qualitycontrol: | NMR1HandHPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
| Excitationmaximum,nm: | 555 |
| ε,L⋅mol−1⋅cm−1: | 150000 |
| Emissionmaximum,nm: | 570 |
| Fluorescencequantumyield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |