Properties:
Related Categories |
Analytical/Chromatography,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
Proteomix HIC Butyl-NP5 Columns, 5 Micron,
Proteomix HIC Butyl-NP5 Columns, Guard Cartridge and Holder, 5 Micron,
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material
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stainless steel hardware
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mfr. no.
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Sepax, 431NP5-4610
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parameter
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6000 psi max. pressure
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80 °C max. temp.
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L × I.D.
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100 mm × 4.6 mm
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impurities
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<10 ppm metals
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matrix
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polystyrene/divinylbenzene particle platform (spherical, highly cross-linked)
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matrix active group
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C4 (butyl) phase
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particle size
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5 μm
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pore size
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nonporous pore size
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operating pH range
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2 - 12
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suitability
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suitable for HPLC
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mode of chromatography
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Hydrophobic interaction (HIC)
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Description:
Analysis Note
Proteomix®?HIC columns are specially designed for high resolution and high efficiency separations of proteins, oligonucleotides and peptides. Utilizing proprietary surface technologies, Proteomix?HIC-NP resin is made of non-porous polystyrenedivinylbenzene (PS/DVB) beads with narrow-dispersed particle size distribution. The PS/DVB bead is modified with alkyl groups or aryl group that provides hydrophobic interaction with analytes. Proteomix?HIC-NP resin is highly rigid and mechanically stable. In comparison to silica based HIC phase media, Proteomix?HIC-NP phases have advantages for biomolecule separations with wide pH range (2-12) and high thermal stability. The nonporous structure and narrow particle distribution offer special selectivity, high resolution separation of proteins such as mAb (monoclonal antibody), ADC (antibody drug conjugate) and related protein fragments, DNA and oligonucleotides. Proteomix?HIC-NP media is applicable at laboratory discovery, laboratory-scale purification and process chromatography for the production of a few mgs to kilogram of proteins.
Compatibility
Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, methanol, or THF.
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Legal Information
Proteomix is a registered trademark of Sepax Technologies