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特別 提示
Duringthelastyears,Cyanine5(ananalogofCy5®)hasbecomeanincredIBLypopularlabelinlifescienceresearchanddiagnostics.Thefluorophorehasitsemissionmaximumintheredregion,wheremanyCCDdetectorsexhibitmaximumsensitivity,andBIOLOGicalobjectsshowlowbackground.Thedyecolorisveryintense,thereforequantitiesassmallas1 nmolcanbedetectedingelelectrophoresisbynakedeye.
ThisCyanine5NHSester(analogtoCy5®NHSester)isareactivedyeforthelabelingofamino-groupsinpeptides,proteins,andoligonucleotides.Thisdyerequiresasmallamountoforganicco-solvent(suchasDMForDMSO)tobeusedinlabelingreactions(pleaseseeourrecommendedprotocolformoredetails).Thisreagentisidealforverycost-efficientlabelingofsolubleproteinsaswellasallkindsofpeptidesandoligonucleotides.Thisreagentalsoworkswellinorganicsolventsforsmallmoleculelabeling.Formoresophisticatedtargetssuchaseasilydegradableproteins,whentheuseofDMForDMSOisundesirable,considerusingwater-solublesulfo-Cyanine5NHSesterwhichdoesnotrequireanyco-solvent,andfeaturesverysimilarfluorescentproperties.
Cyanine5fluorophoreiscompatiblewithvariousinstrumentationincludingmanyfluorescentmicroscopes,imagers,scanners,andfluorescencereaders.AnumberofvariousCyanine5analogsexist-Cyanine5NHSestercanreplaceactivatedestersofCy5®,AlexaFluor647,andDyLight649.
| Appearance: | darkbluepowder |
| Molecularweight: | 616.19 |
| CASnumber: | 1032678-42-4,350686-88-3 |
| Molecularformula: | C36H42ClN3O4 |
| Solubility: | goodinpolar(DMSO,DMF)andchlorinated(DCM,chloroform)organicsolvents,lowsolubilityinwater |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
| Excitationmaximum,nm: | 646 |
| ε,L⋅mol−1⋅cm−1: | 250000 |
| Emissionmaximum,nm: | 662 |
| Fluorescencequantumyield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
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