Boc-protectedCyanine5hydrazidereactivedyeforthelabelingofaldehydesandketones.
Boc-(tert-butoxycarbonyl)protectivegroupcanberemovedwithtrifluoroaceticacid,orotherstrongacid.Reactivehydrazideisformed,whichcanthencouplewithvariouscarbonylgroupsyieldingCyanine5-labeledmolecules.
Generalproperties
| Appearance: | darkbluepowder |
| Molecularweight: | 633.26 |
| Molecularformula: | C37H49ClN4O3 |
| Solubility: | goodindichloromethane,polarorganicsolvents(DMF,DMSO),alcohols,lowinwater |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight. |
Spectralproperties
| Excitationmaximum,nm: | 646 |
| ε,L⋅mol−1⋅cm−1: | 250000 |
| Emissionmaximum,nm: | 662 |
| Fluorescencequantumyield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |