Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species.
Alkaline Phosphatase
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.
Target: MouseHost: DonkeyAntibody Format: F(ab')? Fragment Specificity: IgG (H+L)Minimal Cross Reactivity: Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, Sheep Serum Proteins
Conjugate: Alkaline PhosphataseProduct Category: F(ab')₂ Fragment Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2340780